Transcriptomic Analysis Reveals the Involvement of PpCYP703A2, Pp4CL, and PpABCG26 in Regulating Peach (Prunus persica) Pollen Fertility
YE Mao1,2, LIU Chun-Sheng1,2, LIU Ya-Ting1,2, ZHANG Man1,2, SU Kai1,2, ZHANG Chen-Guang1,2, LI Xiao-Ying1,2, WANG Hai-Jing1,2, XIAO Xiao1,2, ZHANG Li-Bin1,2, YANG Qing3,4, WU Jun-Kai1,2*
1 Horticultural Science and Technology College, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; 2 Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Qinhuangdao 066000, China; 3 The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; 4 Institute of Tree Development and Gene Editing, Beijing Forestry University, Beijing 100083, China
Abstract：Many varieties of peach (Prunus persica) are self-fertile, where normal pollen development is essential for yield, and abnormal pollen development affects self-pollination fertilisation and fruit set. Hence, the study of peach pollen fertility is of great industrial and theoretical importance. The sterile variety 'JiuShuo' (JS) and the fertile variety 'JiuCui' (JC) and their hybrid progeny as test material were used to study pollen fertility genes. Cytological observations indicate that pollen sterility occurred at the mononuclear microspore stage, where the cytoplasm of the tapetum could not be concentrated and degenerated. RNA-seq analysis showed that the pathways associated with the formation of programmed cell death and the outer wall of the pollen layer - sugar metabolism, phenylalanine metabolism, fatty acid biosynthesis and oxidation-reduction were significantly enriched during the tetrad and mononuclear microspore periods. The expression patterns of cytochrome P450 703A2 (PpCYP703A2) (GenBank No. XM_007220109), 4-coumarate-CoA ligase-like 1 (Pp4CL) (GenBank No. XM_007225695), and ABC transporter G family member 26 (PpABCG26) (GenBank No. XM_020565361) were examined in the fertile variety JC, the sterile variety JS, and the JS×JC progeny , using qRT-PCR to detect the expression of related genes, suggest that these three genes were involved in the regulation of pollen fertility. A new genetic resource is provided for further study of peach pollen fertility and a new theoretical basis is developed for further understanding peach pollen sterility.
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