美国红枫叶片转录组分析

doi:10.3969/j.issn.1674-7968.2020.03.007

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摘要美国红枫(Acer rubrum)叶色鲜红美丽,在园林植物中被广泛的应用,同时也常用作防护林树种和风景林树种。为了了解美国红枫转色期叶片中差异表达基因及分子调控途径,本研究对美国红枫转色期叶片进行RNA-Seq测序和分析,共获得unigenes 78 571条,总长度为71 788 869 bp,平均长度913.68 bp。通过与蛋白数据库的比对,48 387条unigenes可获得注释信息。基因本体(Gene Ontology, GO)分析发现注释到的基因涉及53个功能组,其中代谢过程(生物学过程)、细胞(细胞组分)以及催化活性(分子功能)的富集程度较高。KEGG分析发现差异表达基因主要对核糖体代谢等生物学过程进行调节。在叶片转色期间,共筛选出7个与花青素相关的转录因子,主要经丙烷类生物合成、花青素生物合成、类黄酮生物合成等机制介导美国红枫叶色调控。同时发现这7个转录因子在红叶或者花叶中表达量较高,进一步提示其在美国红枫叶片转色期可能发挥重要的作用。本研究结果为探究美国红枫叶色变化的分子机制研究提供了参考。

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	郭聪
	王莹
	谈峰
	陈燕
	李玉娟

关键词 ：美国红枫, 转录组, 叶色变化, 差异表达基因

Abstract：American red maple (Acer rubrum) leaves are bright red and beautiful, and are widely used in garden plants. It is also often used as a shelter forest trees species and a scenic forest trees species. In order to understand the differentially expressed genes and molecular regulatory pathways in American red maple during the color changes of leaves, this study performed RNA-Seq sequencing and analysis on American red maple during the color changes of leaves. The total number of unigenes was 78 571, the total length was 71 788 869 bp, and the average length was 913.68 bp. 48 387 unigenes could be assigned to the protein database for annotation information. Gene Ontology (GO) analysis found that the annotated genes involved 53 functional groups, of which metabolic processes (biological processes), cells (cell components), and catalytic activities (molecular functions) were enriched to a higher degree. KEGG analysis found that differentially expressed genes mainly regulated biological processes such as ribosomal metabolism. During the color change of leaves, 7 kinds of anthocyanin-related transcription factors were selected. And they were mainly through propane biosynthesis, anthocyanin biosynthesis and flavonoid biosynthesis, which were involved in the regulation of American red maple leaf color. Meanwhile, it was found that the expression levels of 7 transcription factors were higher in red leaves or colorful leaves, which further indicated that they might had an important role in the leave color changes of American red maple. The results of this study provide a reference for exploring the molecular mechanism of the change of leaf color in American red maple.

Key words： American red maple Transcriptome Leave color change Differentially expressed gene

收稿日期: 2019-08-13

ZTFLH:

S792

基金资助:江苏省农业自主创新资金项目(CX(19)3121; CX(17)1004)

通讯作者: lyglyj90@sohu.com

引用本文:

郭聪, 王莹, 谈峰, 陈燕, 李玉娟. 美国红枫叶片转录组分析[J]. 农业生物技术学报, 2020, 28(3): 441-454.
GUO Cong, WANG Ying, TAN Feng, CHEN Yan, LI Yu-Juan. Transcriptome Analysis of American Red Maple (Acer rubrum) Leaves. 农业生物技术学报, 2020, 28(3): 441-454.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.03.007 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I3/441

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