Screening of Key Genes for Ovarian Hypoplasia and Analysis of Differential Gene Expression in Mulard Ducks (Cairina moschata)
LI Li1,2, ZHANG Lin-Li1, MIAO Zhong-Wei1, ZHU Zhi-Ming1, QIU Jun-Zhi2, WANG Juan3, XIN Qing-Wu1, ZHENG Nen-Zhu1,*
1 Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3 Ganzhou Agriculture College, Ganzhou 341100, China
Abstract：Mulard duck is an inter-genus hybrid product of Muscovy duck (Cairina moschata) and domestic duck (Anas plalyrhynchas var. domestica),which has strong heterosis but no reproductive ability. In actual production, it is necessary to raise the parents of Mulard ducks, and with the help of artificial insemination technology, the process is cumbersome and the feeding management cost is high, which severely restricts the development of Mulard duck production. Due to the particularity of the sterile traits of Mulard ducks, the analysis of differentially expressed genes in the ovarian tissues of Mulard ducks has not been reported. The information on related genes in the GenBank sequence database is relatively scarce. The genetic basis of infertility needs further research. Transcriptome sequencing (RNA-seq) technology could not only be used for analysis of tissue changes after virus infection, but also for gene mining and functional annotation. In order to explore the molecular mechanism of genes related to ovarian hypoplasia and infertility in Mulard duck, transcriptome sequencing was performed on the ovarian tissues of Mulard ducks and Pekin ducks, the differentially expressed genes related to ovarian differentiation and development were screened, and the genes were annotated with Gene Ontology (GO) and KEGG, and then the qRT-PCR was used to verify the sequencing results. A total of 43.84 Gb clean data and 193 535 Unigenes were obtained in the sequencing. A total of 89 differentially expressed genes related to ovarian development, sex hormone synthesis and germ cell differentiation and development were found between two types of ducks, among them 17 genes were down-regulated, 72 genes were up-regulated in Mulard ducks, Go functional annotation differential genes were enriched in 591 GO terms, in which the significantly enriched calcium-mediated signal transduction was related to germ cell differentiation and development. KEGG analysis showed that differentially expressed genes are enriched in 15 signal pathways, Including glycosides biosynthesis-ganglion series, steroid hormone biosynthesis pathway and tyrosine metabolism related to the synthesis and secretion of sex hormones. The results of qRT-PCR showed that the expression trends of up-regulated and down-regulated genes in Mulard ducks were consistent with the transcriptome sequencing results, indicating that the transcriptome sequencing data was more reliable. This study provides theoretical reference for exploring the differentiation mechanism of the special reproductive system of Mulard ducks.
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