Effects of Low Temperature Treatment on Gene Expression and Flavonoids Biosynthesis Metabolism in Peach (Prunus persica) Leaves
ZHOU Ping1, LIN Zhi-Kai2, GUO Rui1, YAN Shao-Bin1, ZHANG Xiao-Dan4, MA Xin-Yi3, JIN Guang1,*
1 Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2 Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, China; 3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 4 Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana 61801, USA
Abstract：Peach (Prunus persica) is an important economic fruit tree in China, which plays an important role in the development of agricultural economy. Low temperature affects peach growth and development, which is one of the threats to peach production in early spring. In order to explore the low temperature adaptation and cold acclimation mechanism of peach trees in subtropical region, in this study, the changes of transcriptional expression profiles in leaves of peach germplasm 'mx14-1' with low chilling requirement were compared and analyzed, GO functional annotation and KEGG pathway functional enrichment analysis of differentially expressed genes showed that, under low temperature stress, differentially expressed genes in peach leaves were mainly related to stress response, and enriched in secondary metabolite synthesis pathway, involving flavonoids, terpenoids, diphenylheptanone, gingerol and phenylpropane synthesis, linoleic acid metabolism and other pathways. The genes related to flavonoid metabolism were analyzed by qRT-PCR, results showed that the expression of PAL (phenylalanine ammonialyase), C4H (cinnamic acid 4-hydroxylase), 4CL (4-coumarate:CoA ligase), CHS (chalcone synthase), CHI (chalcone isomerase) and F3H (flavanone 3-hydroxylase) gene, which encode key flavonoid biosynthetic enzymes, were significantly up-regulated in 4 ℃ 48 h treated leaves, the results were consistent with those of transcriptome sequencing. The determination of leaf metabolites showed that the contents of flavonols and derivatives related to flavonoid metabolism in peach leaves increased significantly under low temperature stress. 4 ℃ 48 h treatment group and control were treated with -2 ℃, the results showed that the total flavonoids and antioxidant capacity of peach leaves were significantly increased after 4 ℃ low temperature treatment, and the damage decreased at -2 ℃. This study provides a reference for further exploring the molecular mechanism of peach low temperature response and cold resistance.
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