The Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology/Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Abstract：Aquaporins (AQPs) are highly conserved membrane proteins that facilitate the passive movement of water and other small hydrophilic molecules across biological membranes, playing essential roles in the physiological processes of seed germination, flowering, and fruit development, etc. In this study, 28 APQ gene family members (PpAQP1~PpAQP28) were identified from the peach (Prunus persica) genome, which could be categorized into 5 subfamilies, including plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin-26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and uncategorized X intrinsic proteins (XIPs) based on their phylogenetic relationships. Analyses of the chromosomal assignment, gene structures and conserved motifs of these PpAQP members were further performed. Combining with the transcriptome and qPCR detection, tissue-specific expression profiles of these PpAQP genes and their expression patterns at various fruit development stages were analyzed. These results demonstrate the comprehensive expression differentiations of PpAQPs among and within the sub-families, 8 PpAQPs were highly expressed in fruits with their expression high peaks in the early stage of fruit development, suggested their potential roles in the first rapid growth period of fruit development. This research offers a reference for subsequent functional studies of AQPs in fruit development.
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