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2025年8月21日 星期四
农业生物技术学报  2022, Vol. 30 Issue (12): 2363-2375    DOI: 10.3969/j.issn.1674-7968.2022.12.009
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
新西兰兔三个发育阶段骨骼肌的转录组分析
朱翠云, 郑琪, 敬敬, 秦帅奇, 卢嘉妮, 李爽, 凌英会*
安徽农业大学 动物科技学院/地方畜禽遗传资源保护与生物育种安徽省重点实验室,合肥 230036
Transcriptome Analysis of Skeletal Muscles of New Zealand Rabbits (Oryctolagus cuniculus) at Three Developmental Stages
ZHU Cui-Yun, ZHENG Qi, JING Jing, QIN Shuai-Qi, LU Jia-Ni, LI Shuang, LING Ying-Hui*
College of Animal Science and Technology, Anhui Agricultural University/Anhui Provincial Key Laboratory of Genetic Resources Protection and Biological Breeding of Local Livestock and Poultry, Hefei 230036, China
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摘要 信使RNA (messenger RNA, mRNA)在调节骨骼肌发育的复杂动态网络中扮演重要角色。新西兰兔(Oryctolagus cuniculus)从胎儿到成年骨骼肌mRNA的作用尚不清晰。为筛选出与不同阶段新西兰兔骨骼肌发育相关的通路及候选基因,本研究选取新西兰兔2周龄胚胎、6周龄幼兔和6月龄成年兔的腿部肌肉进行转录组测序,分析差异mRNA及其潜在调控作用并通过qPCR进行验证。结果显示,共鉴定出12 845个mRNA,其中1 891个mRNA在3个发育阶段差异表达。胚胎组和幼年组之间有929个差异表达基因(上调393个, 下调536个);胚胎组和成年组有938个差异表达基因(上调449个, 下调489个);幼年组和成年组有24个差异表达基因(上调9个, 下调15个)。GO富集分析显示,胚胎组与幼年组的差异转录本与横纹肌收缩和骨骼肌收缩相关。幼年组和成年组的差异转录本与轴突延伸正调控、细胞生长正调控相关。KEGG分析结果显示,胚胎组与幼年组的差异转录本主要富集在PI3K/AKT信号通路、FoxO信号通路和AMPK信号通路。幼年组和成年组的差异转录本富集到控制肌肉生长发育的cGMP-PKG信号通路和HIF-1信号通路。qPCR验证了随机选取的10个差异基因的表达量与RNA-seq的分析结果一致。在胚胎组和幼年组中获得了ARG2ATPTA2TPM1PRKAG3PRKAA2FoxO3等肌肉发育相关基因,在幼年组和成年组中获得了GOLGA4PFKFB3等肌肉生长相关基因。本研究筛选了与新西兰兔骨骼肌发育的相关基因及相关通路,为后续研究新西兰兔骨骼肌发育提供了一定的理论依据。
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朱翠云
郑琪
敬敬
秦帅奇
卢嘉妮
李爽
凌英会
关键词 新西兰兔骨骼肌转录组测序差异表达基因    
Abstract:Messenger RNA (mRNA) plays an important role in the complex dynamic network regulating skeletal muscle development. The role of New Zealand rabbit (Oryctolagus cuniculus) mRNA from fetus to adult skeletal muscle remains unclear. This study aims to screen for pathways and candidate genes associated with different stages of skeletal muscle development in New Zealand rabbits using transcriptome sequencing technology. In this study, the leg muscles of 2-week-old fetus, 6-week-old child rabbits and 6-month-old adult New Zealand rabbits were selected for transcriptome sequencing, and differential mRNAs and their potential effects were analyzed. The results showed that 12 845 mRNAs were identified, of which 1 891 mRNAs were differentially expressed in 3 stages. There were 929 differentially expressed genes (393 up-regulated, 536 down-regulated) between fetus group and child group, fetus group and adult group have 938 differentially expressed genes (up-regulated 449, down-regulated 489); and 24 differentially expressed genes (9 up-regulated and 15 down-regulated) between the child and adult groups. GO enrichment analysis showed that the differential transcripts between fetus and child groups were associated with transverse muscle contraction and skeletal muscle contraction. KEGG analysis showed that differential transcripts in the fetus and child groups were mainly enriched in PI3K/AKT signaling pathway, FoxO signaling pathway and AMPK signaling pathway. Differential transcripts in the child and adult groups were enriched in the cGMP-PKG signaling pathway and HIF-1 signaling pathway, which control muscle growth and development. The expression of 10 randomly selected differential genes verified by qPCR were consistent with the analysis of RNA-seq. Genes related to muscle development such as ARG2, ATPTA2, TPM1, PRKAG3, PRKAA2 and FoxO3 were obtained in the fetus and child groups, and genes related to muscle growth such as GOLGA4 and PFKFB3 were obtained in the child and adult groups. This study screened the genes and related pathways associated with skeletal muscle development in New Zealand rabbits, which can provide some theoretical basis for the subsequent study of skeletal muscle development in New Zealand rabbits.
Key wordsNew Zealan rabbit (Oryctolagus cuniculus)    Skeletal muscle    Transcriptome sequencing    Differentially expressed genes
收稿日期: 2022-01-24     
ZTFLH:  S829.1  
基金资助:安徽省自然科学基金优秀青年基金(2108085Y11); 安徽省学术与技术带头人后备人选人才项目(2019H206)
通讯作者: * lingyinghui@ahau.edu.cn   
引用本文:   
朱翠云, 郑琪, 敬敬, 秦帅奇, 卢嘉妮, 李爽, 凌英会. 新西兰兔三个发育阶段骨骼肌的转录组分析[J]. 农业生物技术学报, 2022, 30(12): 2363-2375.
ZHU Cui-Yun, ZHENG Qi, JING Jing, QIN Shuai-Qi, LU Jia-Ni, LI Shuang, LING Ying-Hui. Transcriptome Analysis of Skeletal Muscles of New Zealand Rabbits (Oryctolagus cuniculus) at Three Developmental Stages. 农业生物技术学报, 2022, 30(12): 2363-2375.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.12.009     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I12/2363
 
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