Identification and Expression Analysis of CDPK Gene Family in Pecan (Carya illinoinensis) and Chinese Hickory (Carya cathayensis)
ZHAO Juan1,2, ZHU Kai-Kai1,2, FAN Ping-Hua1,2, TAN Peng-Peng1,2, PENG Fang-Ren1,2,*, LI Yong-Rong3
1 Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2 College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 3 Nanjing Green Universe Pecan Science and Technology Co. Ltd., Nanjing 211500, China
Abstract：Calcium-dependent protein kinase (CDPK) is a specific type of Ser/Thr protein kinase to plants, which plays an important role in plant growth, development and stress signal transduction. In order to explore the function of CDPK genes in the growth and development of pecan (Carya illinoinensis) and Chinese hickory (Carya cathayensis), bioinformatics methods were applied to identify the members of CDPK gene family from pecan and Chinese hickory genome. MEGA7.0 software was used to carry on the multiple sequence alignment, classification and phylogenetic tree construction. The gene structures and conserved motifs of CDPK family were also analyzed. The results showed that 28 (CILCDPK1~28) and 25 (CCACDPK1~25) CDPK members were successfully identified in pecan and Chinese hickory, respectively. In order to explore the homologous evolutionary relationship between the two plants and other species, a phylogenetic tree was constructed using CDPKs in pecan, Chinese hickory, Arabidopsis and rice (Oryza sativa). The phylogenetic tree showed that all CDPK proteins could be divided into 4 subgroups. Cis-acting element analysis showed that all CDPK genes contained hormone-response elements, and abscisic acid-response elements were the most abundant. Transcriptome data analysis of pecan and Chinese hickory showed that 3 CILCDPKs and 2 CCACDPKs might play an important role in the 3 key stages of embryonic development. In addition, 3 CDPK genes showed high expression during pecan grafting and healing, which might play important roles in callus and vascular tissue formation of pecan. qPCR analysis showed that CILCDPK1/3/6/11/13/19/25 were up-regulated under drought stress. The present study provides a reference basis for exploring the mechanism of CDPK gene family in plant growth and development and drought stress response.
赵娟, 朱凯凯, 范平桦, 谭鹏鹏, 彭方仁, 李永荣. 薄壳山核桃和山核桃CDPK基因家族的鉴定及表达分析[J]. 农业生物技术学报, 2022, 30(3): 442-456.
ZHAO Juan, ZHU Kai-Kai, FAN Ping-Hua, TAN Peng-Peng, PENG Fang-Ren, LI Yong-Rong. Identification and Expression Analysis of CDPK Gene Family in Pecan (Carya illinoinensis) and Chinese Hickory (Carya cathayensis). 农业生物技术学报, 2022, 30(3): 442-456.
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