Abstract：Myostatin (MSTN) negatively regulates skeletal muscle growth, and the animals carrying the mutated gene all show better growth performance and meat production performance than the normal animals. Whether the significantly increased meat yield will affect the quality of meat is a general concern of consumers. In this study, the MSTN gene-edited cattle (Bos taurus) were used to examine the slaughter traits and meat quality indexes. The results showed that the slaughter rates of MSTN edited cattle and control cattle were (60.83±4.68)% and (55.71±0.53)%, and the net meat rates were (40.63±2.00)% and (37.62±0.79)%, respectively, with significant differences (P<0.05). The meat quality indexes such as pH value, tenderness, water power and nutrition of MSTN-edited cattle were all with no significant differences compared with the control cattle. Gene expressions of longissimus dorsi muscle detected by RNA-Seq and qPCR showed that MSTN mutation resulted in down-regulation of the genes associated with fat synthesis such as Adiponectin, C1Q and collagen domain containing (ADIPOQ), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN), lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ), and stearoyl-CoA desaturase (SCD), decreased the intermuscular fat, and increased the lean meat production. In conclusion, the artificial mutation of MSTN gene can significantly improve the meat production performance of Luxi cattle, not change the meat quality, reduce intramuscular fat deposition, and improve the lean meat production.
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