Effects of Different Storage Conditions on the Content of Chlorophyll and Expression Analysis of Related Genes in Celery (Apium graveolens)
JIA Min, ZHU Sheng-Qi, WANG Ya-Hui, TAN Shan-Shan, LIU Jie-Xia, XIONG Ai-Sheng*
College of Horticulture, Nanjing Agricultural University, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China
Abstract：Celery (Apium graveolens) is an important leafy vegetable with high nutritional values and numerous medicinal functions. Chlorophyll content is one of the important indexes to evaluate the quality of celery during storage after harvest. The influence on the content of chlorophyll in celery under different postharvest environmental conditions is still unclear. In this study, 'Sijixiaoxiangqin' and 'Liuhehuangxinqin' were stored at 3 different storage conditions of room temperature (25 ℃), low temperature (4 ℃) and darkness at room temperature (25 ℃). The chlorophyll contents and the expression levels of metabolism-related genes (AgMPE, AgPPH, AgPAO, AgCAO, AgNOL and AgHCAR) were detected at 0, 6, 24, 30, 48 and 54 h after treatment. The results showed that the average contents of chlorophyll a and chlorophyll b were the highest under low temperature treatment, and total accumulation of chlorophyll was the highest at low temperature storage except for 24 and 30 h, and the peak of total chlorophyll content appeared at low temperature. The total chlorophyll content under darkness at room temperature condition was higher than that under room temperature condition. The transcription level of AgCAO gene changed significantly with the increase of storage time, which was consistent with the accumulation pattern of chlorophyll b. The results indicated that low temperature condition inhibited the degradation of chlorophyll content and could better maintain the chlorophyll content of celery. Low temperature could maintain the nutritional and sensory quality of celery. This study provides a reference for exploring the regulation pattern of chlorophyll accumulation and related genes in celery after harvest.
贾敏, 朱胜琪, 王雅慧, 谭杉杉, 刘洁霞, 熊爱生. 不同贮藏条件对芹菜叶绿素含量的影响及相关基因表达分析[J]. 农业生物技术学报, 2022, 30(6): 1078-1086.
JIA Min, ZHU Sheng-Qi, WANG Ya-Hui, TAN Shan-Shan, LIU Jie-Xia, XIONG Ai-Sheng. Effects of Different Storage Conditions on the Content of Chlorophyll and Expression Analysis of Related Genes in Celery (Apium graveolens). 农业生物技术学报, 2022, 30(6): 1078-1086.
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