Effect of Spermine on Expression of Polyamine Metabolism-related Key Genes and Polyamine Contents in Jejunum and Ileum of Female Geese (Anser cygnoides)
YI Zhi-Xin1,2, JIANG Yi-Long1, LI Lin-Xiang2, JIANG Dong-Mei1, KANG Bo1,*
1 College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; 2 Bazhong Academy of Green Agriculture & Innovative Development, Bazhong 636000, China
Abstract：Spermine accelerates the repair of intestinal mucosal injury, improves activities of intestinal digestive enzymes, polyamine homeostasis, and intestinal function. To research the effects of spermine on key genes related to polyamine metabolism and polyamine contents in the jejunum and ileum in Sichuan white goose (Anser cygnoides), the female geese were intragastrically administered with 5 and 10 mg/kg spermine (body weight). The mRNA expression levels of key genes related to polyamine metabolism and the content of polyamines in female geese in jejunum and ileum tissues were measured by qPCR and high performance liquid chromatography. The results showed that the expression levels of ornithine decarboxylase antizyme inhibitor 2 gene (AZIN2), ornithine decarboxylase antizyme 1 (OAZ1) and OAZ2 genes, and the contents of spermidine and spermine in jejunum of female geese treated with spermine at 5 mg/kg were significantly higher than that in the control group (P<0.05), while expression levels of AZIN1 and acetylpolyamine oxidase gene (APAO) were significantly decreased (P<0.05). The expression levels of AZIN2 and spermine synthase gene (SPMS) in ileum of female geese treated with spermine at 5 mg/kg were significantly higher than that in the control group (P<0.05), while the mRNA expression level of spermidine/spermine-N'-acetyltransferase gene (SSAT) was significantly decreased (P<0.05). The expression levels of AZIN2, OAZ2, ornithine decarboxylase gene (ODC), APAO and spermine oxidase gene (SMO) and spermine content in jejunum of female geese treated with spermine at 10 mg/kg were significantly higher than that in the control group (P<0.05), while the mRNA expression level of spermidine synthase gene (SPDS) and putrescine content were significantly lower (P<0.05), respectively. The expression levels of AZIN2, ODC, SPDS and APAO in ileum of female geese treated with spermine at 10 mg/kg were significantly higher than that in the control group (P<0.05), while the content of putrescine was significantly decreased (P<0.05). These results suggest that exogenous spermine regulates polyamine homeostasis in small intestinal tissues of female geese by mediating the expression levels of polyamine metabolism genes. The effects of spermine on polyamine homeostasis in jejunum and ileum of female geese were different to some extent. This work provides some basic information to polyamine metabolism researches in goose small intestine, and lays a foundation for the further studies on the polyamine metabolism in poultry intestine.
易治鑫, 蒋易龙, 李林祥, 姜冬梅, 康波. 精胺对雌鹅空肠、回肠多胺代谢关键基因的表达及多胺水平的影响[J]. 农业生物技术学报, 2022, 30(8): 1571-1579.
YI Zhi-Xin, JIANG Yi-Long, LI Lin-Xiang, JIANG Dong-Mei, KANG Bo. Effect of Spermine on Expression of Polyamine Metabolism-related Key Genes and Polyamine Contents in Jejunum and Ileum of Female Geese (Anser cygnoides). 农业生物技术学报, 2022, 30(8): 1571-1579.
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