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2025年8月5日 星期二
农业生物技术学报  2022, Vol. 30 Issue (9): 1724-1736    DOI: 10.3969/j.issn.1674-7968.2022.09.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
刺梨CaM基因的鉴定及其在果实中的表达特点
赵熳秋, 南红, 鲁敏*, 安华明
贵州大学 国家林业和草原局刺梨工程技术研究中心,贵阳 550025
Identification of CaM Genes and Its Expression Characteristics in Fruit of Rosa roxburghii
ZHAO Man-Qiu, NAN Hong, LU Min*, AN Hua-Ming
Engineering Technology Research Centre for Rosa roxburghii of National Forestry and Grassland Administration/Guizhou University, Guiyang 550025, China
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摘要 钙调蛋白(calmodulin, CaMs)是植物主要的钙结合蛋白,在植物体内参与广泛的生理过程。本研究从刺梨(Rosa roxburghii)全基因组水平鉴定出2个编码CaM蛋白的基因,分别命名为RrCaM1 (GenBank No. ON886228)和RrCaM2 (GenBank No. ON886229),均含有4个典型的EF-hand功能域。其中RrCaM1基因含有3个内含子,而RrCaM2基因无内含子,分别定位于3号和4号染色体上。系统发育及共线性分析表明,刺梨CaM与白梨(Pyrus bretschneideri)、草莓(Fragaria vesca)之间的亲缘关系较近,RrCaM1RrCaM2可分别聚于2个不同的亚类,且RrCaM1RrCaM2分别与草莓有1个直系同源基因,而RrCaM1与白梨有2个直系同源基因。RNA-Seq与qPCR结果均表明,RrCaM2基因表达量随刺梨果实发育显著下降(P<0.05),RrCaM1则相反。顺式作用元件预测RrCaM1的启动子区包含激素、光等8类响应元件,而RrCaM2缺少响应赤霉素、低温、干旱等的顺式作用元件;外源激素处理的结果证实,2个成员对水杨酸(salicylic acid, SA)、脱落酸(abscisic acid, ABA)和赤霉素(gibberellin, GA3)等外源激素处理呈现不同的表达模式。钙调蛋白抑制剂(chlorpromazine, CPZ)处理显著抑制RrCaM表达并降低了刺梨果实维生素C (ascorbate, AsA)的合成,且与AsA合成相关基因(L-galactose phosphatase, RrGPP)和AsA循环基因(dehydroascorbate reductase, RrDHAR)的表达量呈显著正相关(P<0.05),提示其可能参与了刺梨果实AsA积累的调控。本研究发现,RrCaMs基因家族在刺梨果实发育、AsA积累与激素响应中具有潜在的重要作用,为该基因家族的功能研究提供了参考。
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赵熳秋
南红
鲁敏
安华明
关键词 刺梨钙调蛋白(CaMs)基因表达维生素C (AsA)    
Abstract:Calmodulin (CaMs) are the primary calcium-binding proteins in plants which control diverse cellular processes, including plant development and hormonal response. In this study, a genome-wide analysis conducted in Rosa roxburghii and identifified 2 RrCaM-encoding genes named RrCaM1 and RrCaM2. The RrCaM1 contains 3 introns while the RrCaM2 gene has no introns. Both of the RrCaMs contained 4 typical EF-hands. The RrCaM1 and RrCaM2 genes were located on the 3rd and 4th chromosome, repectively. The phylogenetic relationship and synteny analyses showed that RrCaM1 and RrCaM2 were clustered as 2 different subgroups. Besides, RrCaM1 and RrCaM2 shared one orthologous duplication pair with Fragaria vesca, respectively. RrCaM1 and Pyrus bretschneideri had 2 orthologous duplication pairs. CaMs genetic relationship among R. roxburghii, P. bretschneideri and Fragaria vesca was close. The results of RNA-Seq and qPCR indicated that the expression of RrCaM2 gene decreased significantly with the fruit development of R. roxburghii, while RrCaM1 was the opposite. Cis-acting element prediction and qPCR analysis showed that the promoter region of RrCaM1 contained 8 response elements such as hormone and light, while RrCaM2 lacked cis-acting elements responsive to gibberellin (GA3), low temperature and drought, which also showed completely different response patterns to salicylic acid (SA), abscisic acid (ABA), and GA3 treatments.The vitamin C (ascorbate, AsA) content and RrCaMs gene expression of R. roxburghii fruit were significantly decreased by chlorpromazine (CPZ) treatment (P<0.05). RrCaMs had a significant positive correlation with the expression of the L-galactose phosphatase (GPP) gene in AsA biosynthesis pathway and the dehydroascorbate reductase (DHAR) gene in recycling pathway. It indicates that it may be involved in the regulation of AsA accumulation in R. roxburghii fruit. This study found that the RrCaMs gene family played potentially important roles in fruit development, AsA accumulation and hormone response of R. roxburghii, and provides reference for the functional study of this gene family.
Key wordsRosa roxburghii    Calmodulin (CaMs)    Gene expression    Ascorbate (AsA)
收稿日期: 2021-10-13     
ZTFLH:  S661  
基金资助:贵州省科技计划项目(黔科合基础[2020]1Y113); 国家自然科学基金(U1812401)
通讯作者: *mlv@gzu.edu.cn   
引用本文:   
赵熳秋, 南红, 鲁敏, 安华明. 刺梨CaM基因的鉴定及其在果实中的表达特点[J]. 农业生物技术学报, 2022, 30(9): 1724-1736.
ZHAO Man-Qiu, NAN Hong, LU Min, AN Hua-Ming. Identification of CaM Genes and Its Expression Characteristics in Fruit of Rosa roxburghii. 农业生物技术学报, 2022, 30(9): 1724-1736.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.09.007     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I9/1724
 
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