Abstract：miR-206 is one of the miRNAs previously screened by our group by whole-transcriptome sequencing which showed significant differences in high and low residual feed intake in beef cattle (Bos taurus). Cattle miR-206 was used as a study target to analyze the affinity of miR-206 among species, and the potential target genes of bovine miR-206 were predicted by online software such as TargetScan and miRWalk. GO and KEGG enrichment analysis of the target genes were performed. The expression pattern of miR-206 in cattle tissues was detected by qPCR. The results showed that cattle miR-206 was most closely related to goat (Capra hircus), followed by horse (Equus caballus), and the most distantly related to Xenopus laevis. Among the potential target genes of miR-206 related to muscle development were histone deacetylase 4 (HDAC4), heat shock protein family D member 1 (HSPD1), and fibronectin (FN1). The functional enrichment analysis revealed that the predicted target genes were significantly enriched in MAPK, PI3K-AKT and TNF, which were signaling pathways related to muscle development. qPCR results showed that miR-206 expression was the highest in the leg and longest dorsal muscles of adult cattle and was extremely significant higher than that in other tissues (P<0.01), followed by liver, and the lowest was in the duodenum and subcutaneous. Compared with newborn calves, miR-206 expression was extremely significant down-regulated in subcutaneous fat and up-regulated in dorsal longest muscle and leg muscle (P<0.01) in adult cattle. The results of this study will provide a reference for further investigation of the role of miR-206 in the growth and development of cattle muscle.
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