State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources/ Guangxi Key Laboratory of Sugarcane Biology/ Guangxi Colleges and Universities Key Laboratory of Crop Cultivation and Tillage/ National Demonstration Center for Experimental Plant Science Education, Guangxi University, Nanning 530004, China
Abstract：Gene editing technology is one of the modern breeding technologies for stable and accurate modification of target genes. CRISPR/Cas9 (clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9) system is the most widely used gene editing technology available today.It recognizes target sites of target genes to break the double-stranded DNA and activates DNA damage repair pathway, so as to achieve accurate gene editing. Now CRISPR/Cas9 has been widely used in rice (Oryza sativa), corn (Zea mays),peanut (Arachis hypogaea) and other important crops. In addition, it can be applied to polyploid crops such as wheat (Triticum aestivum), cotton (Gossypium hirsutum) and soybean (Glycine max) which are thought difficult to be edited. In this paper, the principle of CRISPR/Cas9 mediated gene editing system, its application in polyploid crops and existing problems are discussed on the basis of summarizing some achievements. Meanwhile, suggestions for improving gene editing efficiency are also put forward, hoping to provide reference for future research.
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