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2025年8月14日 星期四
农业生物技术学报  2022, Vol. 30 Issue (7): 1279-1289    DOI: 10.3969/j.issn.1674-7968.2022.07.005
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
胁迫下海马齿生理指标变化及相关基因表达分析
李雨欣1, 罗秀丽1, 张婷婷1, 康宇乾1, 王鹏1, 江行玉2, 周扬1*
1 海南大学 园艺学院/海南省热带园艺作物品质调控重点实验室,海口 570228;
2 海南大学 热带作物学院/海南省耐盐作物生物技术重点 实验室,海口 570228
Physiological Changes and Related Gene Expression Analysis of Sesuvium portulacastrum Under Salt Stress
LI Yu-Xin1, LUO Xiu-Li1, ZHANG Ting-Ting1, KANG Yu-Qian1, WANG Peng1, JIANG Xing-Yu2,ZHOU Yang1*
1 School of Horticulture/Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Hainan University, Haikou 570228, China;
2 School of Tropical Crops/Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops, Hainan University, Haikou 570228, China
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摘要 盐胁迫是限制植物生长发育的主要非生物胁迫之 一 。 本研究以盐生植物海马齿 (Sesuvium portulacastrum)为研究对象 ,分析海马齿幼苗在 400 和 800 mmol/L NaCl 处理 0、6、12、24、48 和 72 h 后 ,其 根和叶生理指标变化及相关耐盐基因的表达情况。结果表明:在 400 mmol/L NaCl 胁迫下,海马齿幼苗在 胁迫初期出现萎蔫现象,随着胁迫时间的延长,海马齿幼苗的生长逐渐稳定;在 800 mmol/L NaCl 胁迫下, 海马齿幼苗随着胁迫时间的延长出现萎蔫并最终死亡 ;叶和根中超氧化物歧化酶(superoxide dismutase, SOD)、抗坏血酸过氧化物酶(ascorbate peroxidase, APX)、过氧化物酶(peroxidase, POD)的活性在 12 h 内显 著增加,可溶性糖和脯氨酸积累量增加较明显,此后酶活性和渗透调节物质含量均降低。相对电导率呈 先升高后下降的趋势。400 mmol/L NaCl 胁迫下 ,海马齿中耐盐相关基因质膜 Na+/H+ 逆转运蛋白基因 (salt overly sensitive 1, SOS1)、蛋白激酶基因(CBL-interaccting protein kinase 8, CIPK8)、类似钙调磷酸酶 B 亚 基 蛋 白 基 因 (calcineurin B-like 10, CBL10) 与 质 膜 H+-ATPase 基 因 (plasma membrane H+-ATPase gene, AHA1)在根和叶中均呈上调表达,在 800 mmol/L NaCl 胁迫下,SpSOS1、SpCIPK8、SpCBL10 和 SpAHA1 基因 在海马齿叶中的表达量在处理后 12 h 达到最高 ,为同时间 400 mmol/L NaCl 胁迫下基因表达量的 6.80、124.32、25.93 和 9.52 倍。本研究为进一步研究植物在盐胁迫下生理指标及相关基因表达调控规律提供 理论依据。
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李雨欣
罗秀丽
张婷婷
康宇乾
王鹏
江行玉
周扬
关键词 海马齿盐胁迫生理变化基因表达    
Abstract:Salt stress is one of the main abiotic stresses that restrict plant growth and development. In this study, the halophyte Sesuvium portulacastrum seedlings were treated with 400 and 800 mmol/L NaCl for 0, 6,12, 24, 48 and 72 h, and the changes of physiological indexes in roots and leaves and the expression level of salt-related genes were analyzed. The results showed that Sesuvium portulacastrum seedlings withered at the beginning of stress, while the growth gradually stabilized with the extension of stress time when treated with 400 mmol/L NaCl. While the seedlings wilted and died gradually with the extension of stress time under 800 mmol/L NaCl. The activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD) in leaves and roots all increased significantly under 12 h treatment. The accumulation of soluble sugar and proline increased obviously, and then the enzyme activity and osmotic regulation substances contents decreased. The relative conductivity increased first and then decreased. Four salt-tolerant genes were selected for real-time fluorescence quantitative verification. The expression levels of salt-related genes, plasma membrane Na+/H+ antiporter gene salt overly sensitive 1 (SOS1), protein kinase gene CBL-interaccting protein kinase 8 (CIPK8), Calcineurin B-like 10 (CBL10) and plasma membrane H+-ATPase gene (AHA1), were up- regulated in the roots and leaves under salt stress. The expression levels of SpSOS1, SpCIPK8, SpCBL10 and SpAHA1 genes under 800 mmol/L NaCl reached the highest at 12 h after treatment, and the expressions were 6.80, 124.32, 25.93 and 9.52 times of those under 400 mmol/L NaCl at the same time. This study provides a theoretical basis for the study of physiological indexes and related gene expression regulation of plants under salt stress.
Key wordsSesuvium portulacastrum    Salt stress    Physiological change    Gene expression
收稿日期: 2021-09-16     
ZTFLH:  S-3  
基金资助:国家重点研发计划(2018YFE0207203-2);海南省自然科学基金(318QN189);海南省教育厅项目(Hnky2021-19)
通讯作者: *zhouyang@hainanu.edu.cn   
引用本文:   
李雨欣, 罗秀丽, 张婷婷, 康宇乾, 王鹏, 江行玉, 周扬. 胁迫下海马齿生理指标变化及相关基因表达分析[J]. 农业生物技术学报, 2022, 30(7): 1279-1289.
LI Yu-Xin, LUO Xiu-Li, ZHANG Ting-Ting, KANG Yu-Qian, WANG Peng, JIANG Xing-Yu,ZHOU Yang. Physiological Changes and Related Gene Expression Analysis of Sesuvium portulacastrum Under Salt Stress. 农业生物技术学报, 2022, 30(7): 1279-1289.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.07.005     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I7/1279
 
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