Effects of Melatonin on the Sprout Tumble Under High Temperature and DNA Methylation in Pinellia ternata
CUI Wan-Ning1,2, YANG Jin-Rong1,2, LIU Meng-Meng1,2, HAN Lei1,2, LIU Xiao1,2, YOU Qian1,2, ZHAO Feng-Lan1,2, XUE Tao1,2, ZHU Yan-Fang1,2,*, XUE Jian-Ping1,2,*
1 College of Life Sciences, Huaibei Normal University, Huaibei 235000, China; 2 Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, Huaibei 235000, China
Abstract：Melatonin is widely involved in the regulation of plant stress response, however, the function and regulatory mechanism of melatonin in the sprout tumble of Pinellia ternata under high-temperature are unclear. In this study, the effect of melatonin treatment on the rate of sprout tumble in P. ternata were compared and the corresponding genomic methylation variation in P. ternata were analyzed based on methylation-sensitive amplification polymorphism (MSAP). The results showed that both 50 and 100 μmol/L melatonin significantly reduced the sprout tumble rate of P. ternata. And the effect of 50 μmol/L melatonin treatment on the methylation variation of the P. ternata genome was analyzed. The total methylation rate of the control group was 56.97% and 45.38% at 0 and 6 h high temperature stress, respectively, and the methylation rate of 50 μmol/L melatonin-treated group was 52.11% at 6 h high temperature stress, indicated that melatonin treatment generally delayed the high temperature stress-induced demethylation of the genome. The analysis of differential methylation bands revealed that melatonin treatment mainly affected the modification of methylation in the mitochondrial and chloroplast genomes of P. ternata. Additionally, P. ternata catalase 2 (PtCAT2) was demethylated under melatonin treatment, and the gene expression was up-regulated, indicated that melatonin could regulate the accumulation of reactive oxygen species through methylation modification, and then participated in the regulation of sprout tumble in P. ternata under high temperature stress. This study explored the function of melatonin on the sprout tumble of P. ternata under high-temperature, and analyzed its possible regulatory pathways from an epigenetic perspective, providing a reference for the study of the regulation mechanism of the sprout tumble of P. ternata.
崔婉宁, 杨金荣, 刘梦梦, 韩磊, 刘晓, 尤倩, 赵丰兰, 薛涛, 朱艳芳, 薛建平. 褪黑素对半夏高温倒苗及DNA甲基化的影响[J]. 农业生物技术学报, 2023, 31(3): 500-508.
CUI Wan-Ning, YANG Jin-Rong, LIU Meng-Meng, HAN Lei, LIU Xiao, YOU Qian, ZHAO Feng-Lan, XUE Tao, ZHU Yan-Fang, XUE Jian-Ping. Effects of Melatonin on the Sprout Tumble Under High Temperature and DNA Methylation in Pinellia ternata. 农业生物技术学报, 2023, 31(3): 500-508.
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