Polymorphisms and Haplotypes of MTNR1A Gene and Their Association with Litter Size in Sheep (Ovis aries)
WANG Ming-Yuan1,2, ZHU Meng-Ting1, SHAO Yan-Yan1, YANG Yong-Lin2, YU Qian2, Nijiati·Xiakaier3, ZHAO Zong-Sheng1,*, YANG Hua2,*
1 College of Animal Science and Technology, Shihezi University, Xinjiang 832000, China; 2 State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China; 3 Shihezi Animal Health Supervision Institute of the 8th division of Xinjiang production and Construction Corps, Shihezi 832000, China
Abstract：Melatonin receptor 1A (MTNR1A) is an important factor affecting the seasonal estrus and litter size in sheep (Ovis aries). To study the correlation of MTNR1A gene with litter size in Chinese Merino (Xinjiang Junken type) and Prolific Suffolk sheep, In this work, PCR, sequencing and restriction fragment length polymorphism (RFLP) techniques were used to detect the SNPs and genotypes in exon 2 of the MTNR1A gene. Linkage disequilibrium analysis was used to construct haplotypes. The association of MTNR1A gene polymorphisms and haplotypes with litter size was analyzed by general linear model (GLM) in two sheep groups. The results showed that there were 3 SNPs (G735A, G753A and C845A), each with three genotypes, were detected in two sheep breeds. SNP1 and SNP2 were synonymous mutations, SNP3 was a missense mutation with a single A to C substitution at nucleotide position 845 resulting in an alanine replacing an aspartic acid. The genotype distribution and allele frequencies of three SNPs in two sheep groups were in Hardy-Weinberg equilibrium and moderate polymorphisms. The associated analysis showed that G735A and G753A loci were extremely significantly associated with average litter size in sheep (P<0.01), the litter size of GG genotype was significantly higher than those of AG and AA genotypes (P<0.01) in two loci. The C845A loci was significantly associated with litter size in sheep (P<0.05), the litter size of CC genotype was significantly higher than those of CC and CG genotypes (P<0.05). Four haplotypes were constructed for three SNPs of MTNR1A gene. The different haplotypes were extremely significantly correlated with litter size in sheep (P<0.01), and the H1 haplotype was significantly correlated with higher average litter size than H4 (P<0.01). The results showed that MTNR1A gene was closely related to litter size in Chinese Merino (Xinjiang Junken type) and Prolific Suffolk sheep. H1 was the main haplotype of high litter size of sheep, G735A, G753A and C845A loci could be used as useful genetic markers in marker assisted selection for breeding of fecundity sheep.
王明远, 朱梦婷, 邵焱焱, 杨永林, 余乾, 尼加提·夏开尔, 赵宗胜, 杨华. 绵羊MTNR1A基因多态性及其单倍型与产羔数的相关性[J]. 农业生物技术学报, 2021, 29(7): 1303-1312.
WANG Ming-Yuan, ZHU Meng-Ting, SHAO Yan-Yan, YANG Yong-Lin, YU Qian, Nijiati·Xiakaier, ZHAO Zong-Sheng, YANG Hua. Polymorphisms and Haplotypes of MTNR1A Gene and Their Association with Litter Size in Sheep (Ovis aries). 农业生物技术学报, 2021, 29(7): 1303-1312.
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