Imprinting Identification and Methylation Analysis of Bovine (Bos taurus) KLF14 and PDE10A Genes
DONG Yan-Qiu1, JIN Lan-Jie1, YANG Xin-Yi1, LI Dong-Jie2, HUO Hao-Nan1, SU Hong3, LI Shi-Jie1*
1 College of Life Sciences, Hebei Agricultural University, Baoding 071000, China; 2 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 3 Baoding University of Technology, Baoding 071000, China
Abstract：In mammals, imprinted genes play key role in placental development and fetal growth. Changes in the expression of imprinted genes can lead to abnormal growth and development in mammals and cause diseases through various physiological responses and signaling pathways. KLF14 (Kruppel like factor 14) and phosphodiesterase 10a (PDE10A) genes were identified as imprinted genes in human (Homo sapiens) and mouse (Mus musculus). At present, the imprinting studies on KLF14 and PDE10A in cattle (Bos taurus) have not been reported. In this study, the expression of KLF14 and PDE10A genes in bovine heart, liver, spleen, lung, kidney, brain and placenta were firstly analyzed by RT-PCR. The results showed that KLF14 gene was only detected in the placenta; PDE10A gene was expressed in all tested tissues. The allelic expression status of KLF14 and PDE10A genes were further analyzed by direct sequencing of RT-PCR products based on SNP genomic DNA, KLF14 gene was identifiied to be a maternally imprinted gene in bovine placenta. PDE10A gene showed biallelic expression in placenta ant other tissues. PDE10A gene was non-imprinted in bovine. The methylation status of the promoter region of KLF14 gene was analyzed by sulfite sequencing method, hypomethylation were found in both bovine placenta and sperm, and there was no differentially methylated region, which indicated that the methylation modification of the promoter region of KLF14 gene might not be involved in regulating the imprinted expression of KLF14 gene. The above results suggest that bovine KLF14 gene is a placenta-specific maternally imprinted gene and bovine PDE10A gene is non-imprinted gene. This study provides a reference for further study of the function of the bovine KLF14 and PED10A.
董艳秋, 靳兰杰, 杨欣怡, 李冬杰, 霍浩楠, 苏红, 李世杰. 牛KLF14和PDE10A基因的印记鉴定和甲基化分析[J]. 农业生物技术学报, 2023, 31(4): 776-783.
DONG Yan-Qiu, JIN Lan-Jie, YANG Xin-Yi, LI Dong-Jie, HUO Hao-Nan, SU Hong, LI Shi-Jie. Imprinting Identification and Methylation Analysis of Bovine (Bos taurus) KLF14 and PDE10A Genes. 农业生物技术学报, 2023, 31(4): 776-783.
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