Analyses of Allele Expression and DNA Methylation Status of Bovine (Bos taurus) ZC3H12C Gene in Tissues and Placenta
ZHAO Guang-Wu1, LIU Xiao-Qian1, YANG Zi-Yi2, GU Shu-Kai1, YANG Xin-Yi1, MENG Shi-Xiong1, ZHANG Cui1,*, LI Shi-Jie1,*
1 College of Life Science, Hebei Agricultural University, Baoding 071001, China; 2 College of Food Science, Hebei Agricultural University, Baoding 071001, China
Abstract：Genomic imprinting is an epigenetic phenomenon that causes genes to be monoallelically expressed dependent on the parent origin. In mammalian, imprinted genes play an important role in placental and embryonic development, as well as in the growth of individuals. The zinc finger CCCH-type containing 12C (ZC3H12C) gene encodes a transcription factor containing a single CCCH-zinc finger domain and is involved in regulating a variety of human immune diseases. ZC3H12C gene was first identified imprinted in the human (Homo sapiens) placenta, but its imprinting status has not been established in cattle (Bos taurus). In this study, the structure of the bovine ZC3H12C gene was first analyzed. Then by the method of comparing genomic DNA and cDNA amplification products based on single nucleotide polymorphism (SNP), the allele expression of ZC3H12C gene was analyzed in bovine tissue. The results showed that the ZC3H12C gene exhibited monoallelic expression in all 6 tested tissues, including heart, liver, spleen, lung, kidney, and brain, but they showed biallelic expression in the bovine placenta. The methylation status of ZC3H12C gene were analyzed by sulfite sequencing method. A differentially methylated region was found in intron 1 of ZC3H12C X2 spliced transcript, but in the placenta this region showed hypormethylated. These results suggesting that the monoallele expression of the ZC3H12C gene in bovine tissues is regulated by DNA methylation modification. These results provide valuable information on the further study of the function of ZC3H12C gene.
赵广武, 刘晓倩, 杨子怡, 谷书凯, 杨欣怡, 孟世雄, 张萃, 李世杰. 牛ZC3H12C基因在组织和胎盘中的等位基因表达及DNA甲基化状态分析[J]. 农业生物技术学报, 2021, 29(1): 58-66.
ZHAO Guang-Wu, LIU Xiao-Qian, YANG Zi-Yi, GU Shu-Kai, YANG Xin-Yi, MENG Shi-Xiong, ZHANG Cui, LI Shi-Jie. Analyses of Allele Expression and DNA Methylation Status of Bovine (Bos taurus) ZC3H12C Gene in Tissues and Placenta. 农业生物技术学报, 2021, 29(1): 58-66.
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