联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月8日 星期五
农业生物技术学报  2020, Vol. 28 Issue (9): 1595-1603    DOI: 10.3969/j.issn.1674-7968.2020.09.008
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
QPCT基因为母源等位基因表达的印记基因
谷书凯1, 刘晓倩1, 李俊良1, 陈玮娜2, 张萃1, 李冬杰3, 李世杰1, *
1 河北农业大学 生命科学学院,保定 071001;
2 河北大学 中医系,保定 071001;
3 河北科技大学 生物科学与工程学院,石家庄 050018
QPCT is An Imprinted Gene with Maternal Allele Expression in Cattle (Bos taurus)
GU Shu-Kai1, LIU Xiao-Qian1, LI Jun-Liang1, CHEN Wei-Na2, ZHANG Cui1, LI Dong-Jie3, LI Shi-Jie1, *
1 College of Life Science, Hebei Agricultural University, Baoding 071001, China;
2 Department of Traditional Chinese Medicine, Hebei University, Baoding 071001, China;
3 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
全文: PDF (6582 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 基因组印记是哺乳动物中存在的一种单等位基因表达的表观遗传学现象。基因组印记与动物的生长发育以及疾病的发生有密切联系。谷氨酰胺肽环转移酶(glutaminyl-peptide cyclotransferase, QPCT)参与蛋白质的翻译后修饰,与人类(Homo sapiens)多种精神疾病有关。Qpct基因在小鼠(Mus musculus)中为胎盘特异的父源印记基因,而在牛(Bos taurus)中的印记状态尚未有研究报道。本研究旨在揭示QPCT基因在牛中的印记状态以及DNA甲基化修饰在调控QPCT基因印记中的可能作用。首先应用基于单核苷酸多态的反转录PCR (reverse transcription PCR, RT-PCR)产物直接测序法对QPCT基因在牛6个组织(心脏, 肝脏, 脾脏, 肺脏, 肾脏和大脑)和胎盘中的等位基因表达状态进行分析,发现QPCT基因在被检测的6个组织和胎盘中均为单等位基因表达。通过分析胎盘父本和母本的基因型,确定QPCT在牛的胎盘中为母源等位基因表达。进一步通过亚硫酸氢盐测序法分析QPCT基因启动子区的CpG岛在牛胎盘和组织中的甲基化状态,发现被检测区域的55个CpG位点在胎盘和组织中均呈现轻甲基化,表明QPCT基因启动子区的DNA甲基化修饰不参与调控QPCT基因在牛中的印记表达。本研究可为深入探讨QPCT基因的功能提供参考依据。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
谷书凯
刘晓倩
李俊良
陈玮娜
张萃
李冬杰
李世杰
关键词 谷氨酰胺肽环转移酶基因(QPCT)印记状态DNA甲基化    
Abstract:Genomic imprinting is an epigenetics phenomenon in mammals that leads to genes expressed from only one of the two parental copies. Imprinted genes play important roles in mammalian growth and development and affect the occurrence of diseases. Glutaminyl-peptide cyclotransferase (QPCT) has been found to be involved in post-translational modification of proteins and implicated in various mental disorders in human (Homo sapiens). Qpct is a placental-specific paternally imprinted gene in mouse (Mus musculus), but its imprinting status in cattle (Bos taurus) is still unknown. The aim of this study was to identify the imprinting status of QPCT gene in cattle and to determine whether DNA methylation would play a role in QPCT imprinting. The allele expression status of QPCT gene in bovine placenta and 6 somatic tissues (heart, liver, spleen, lung, kidney and brain) were analyzed by direct sequencing of reverse transcription PCR (RT-PCR) products based on single nucleotide polymorphisms (SNP). Results indicated that QPCT showed monoallelic expression in all the 6 tissues and placentas. QPCT showed maternal allele expression in bovine placentas by analyzing the genotypes of parental genomic DNA. The methylation status of CpG-enriched region in QPCT promoter were analyzed in bovine placentas and somatic tissues using bisulfite sequencing method, and no differentially methylated regions were detected in 55 CpG sites. The above results indicated that the DNA methylation modification in the promoter region of QPCT gene might not be involved in regulating the expression of QPCT gene in cattle, which would provide valuable information for further study of the function of QPCT gene.
Key wordsCattle    Glutaminyl-peptide cyclotransferase gene (QPCT)    Imprinting status    DNA methylation
收稿日期: 2020-03-01     
ZTFLH:  S823  
  S813.3  
通讯作者: * ,lishijie20005@163.com   
引用本文:   
谷书凯, 刘晓倩, 李俊良, 陈玮娜, 张萃, 李冬杰, 李世杰. 牛QPCT基因为母源等位基因表达的印记基因[J]. 农业生物技术学报, 2020, 28(9): 1595-1603.
GU Shu-Kai, LIU Xiao-Qian, LI Jun-Liang, CHEN Wei-Na, ZHANG Cui, LI Dong-Jie, LI Shi-Jie. QPCT is An Imprinted Gene with Maternal Allele Expression in Cattle (Bos taurus). 农业生物技术学报, 2020, 28(9): 1595-1603.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.09.008     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I9/1595
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn