Expression Analysis of MTR1a Gene in Hypothalamic-pitutary-ovarian Axis During Estrus Cycle in Ganjia Tibetan Sheep (Ovis aries)
YANG Da-Peng1, HE Yu-Qin1,*, GE Wen-Bo1, ZHANG Quan-Wei1, HUADAO Cai-Rang2, WANG Xia1, ZHOU Kai- Ren1, CHEN Wei-Gang1
1 Laboratory of Animal Physiology, College of Life Science and Technology, College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 Ganjia Township Animal Husbandry and Veterinary Workstation of Xiahe County in Gannan Tibetan Autonomous Prefecture, Xiahe 747199, China
Abstract：Melatonin is a steroid hormone secreted by the pineal gland. Its receptor binds to the hypothalamic-pituitary-ovarian axis (HPO) of female animals and plays a role in regulating the seasonal reproduction of animals. To describe the expression pattern of the HPO axis of the estrus cycle melatonin receptor 1a (MTR1a) in Ganjia Tibetan sheep (Ovis aries), 32 healthy Ganjia Tibetan sheep were selected in the erotic cycles, and detected the differential expression of melatonin receptor 1a (MTR1a) in Ganjia Tibetan sheep hypothalamus, pituitary and ovarian tissues of plateau in estrous cycle by using qRT-PCR assays. The results showed that MTR1a was expressed in HPO tissues with entire estrous cycles. In the pre-estrus, estrus, late estrus, and inter-temporal hypothalamic tissue of Ganjia Tibetan sheep, the relative expression of MTR1a gene in hypothalamic tissue was the highest in proestrus, which was significantly higher than that in estrus and late estrus (P<0.01). At the same period, the relative expression of MTR1a gene in pituitary tissue was the highest in diestrus, which was significantly different from estrus (P<0.05); The relative expression of MTR1a in ovarian tissue was the highest in proestrus, which was significantly higher than the other three periods (P<0.01). Taken together, this study found that MTR1a in different stages with estrous cycle was expressed in HPO tissues, and the expression levels had dynamic variation. These results indicated that MT had a regulatory role for Tibetan sheep reproductive activities. This study provides a scientific basis for MT regulation of sheep reproductive performance in molecular level.
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