Bovine (Bos taurus) ANO1 Gene is a Placental-specific Paternal Imprinting Gene
GU Shu-Kai1, LI Jun-Liang1, CHEN Wei-Na1, 2, ZHANG Cui1, XU Da1, LI Dong-Jie3, LI Shi-Jie1, *
1 College of Life Science, Hebei Agricultural University, Baoding 071001, China; 2 Department of Traditional Chinese Medicine, Hebei University, Baoding 071001, China; 3 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
Abstract：In mammals, genomic imprinting is an epigenetic phenomenon by which certain genes are expressed from one allele, in a parent-of-origin-specific manner. ANO1 (Anoctamin 1) protein is a subunit of calcium-activated chloride channel. Overexpression of ANO1 gene is associated with a variety of human (Homo sapiens) cancers. In human, ANO1 gene exhibits biallelic expression in embryos and adult tissues, but shows maternal allele expression in placenta. At present, the imprinting status and regulation mechanism of ANO1 gene in bovine (Bos taurus) have not been reported. In this study, in order to analyze the imprinting status of ANO1 gene in cattle, SNP was first found on ANO1 gene by direct sequencing of PCR products, and then reverse transcription-PCR (RT-PCR) was performed on RNA derived from tissues and placentas of heterozygous cattle. Direct sequencing showed that the imprinting status of ANO1 gene in cattle was placenta-specific imprinting and maternal allele-specific expression. The specific methylation status of the promoter region of ANO1 gene in bovine placenta, bovine sperm and adult bovine tissues was further analyzed by sulfite sequencing. It was found that the promoter methylation of ANO1 gene did not participate in the gene imprinting expression. The results showed that bovine ANO1 gene was a placenta-specific paternal imprinting gene, which provides a reference for further study of the relationship between ANO1 gene expression and related diseases.
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