Abstract：Rumen is an important digestive organ of ruminants, early supplementation with starter can meet the nutritional needs of rapid growth of lambs (Ovis aries) and promote the early development of rumen. This study was conducted to investigate the effects of supplementation with starter on gene expression in rumen tissues of lambs to clarify the possible mechanism of starter on rumen development. Twenty-two healthy and disease-free male Hu lambs (birth weight=(3.65±0.49) kg) were randomly divided into 2 groups, control group and supplementary feeding group. Control group was fed with milk replacer, while supplementary feeding group was fed with milk replacer and starter. At the age of 28 days, rumen pouch tissues were collected for transcriptome sequencing analysis. The result showed that 61.70 and 58.95 Gb of clean reads were obtained in control group and supplementary feeding group, respectively, and the comparison efficiency with the reference genome was 87.85%~89.45%. Compared with the control group, 868 differentially expressed genes were screened after supplementary feeding, among which 464 genes were up-regulated and 404 genes were down-regulated. And it was mainly concentrated in GO functions such as enzyme activity, ion channel and membrane transport and KEGG signaling pathways such as steroid hormone biosynthesis, arachidonic acid metabolism, peroxisome proliferator-activated receptor (PPAR) signaling pathway, regulation of lipolysis in adipocytes and cyclic adenosine monophosphate (cAMP) signaling pathway. Correlation analysis showed that recombinant IQ motif containing GTPase activating protein 2 (IQGAP2), recombinant solute carrier family 16, member 1 (SLC16A1), ankyrin repeat domain-containing protein 45 (ANKRD45), 3-hydroxy-3-methylglutaryl-CoA, synthase 2 (HMGCS2), phosophoenolpyruvate carboxykinase 2 (PCK2) were significantly positively correlated with the length of rumen papilla, the concentration of acetate, propionate, butyrate and total volatile fatty acid (TVFA)(P<0.05), while ceramide synthase 3 (CERS3), recombinant keratin 4 (KRT4), solute carrier family 22A17 (SLC22A1), recombinant insulin like growth factor binding protein 6 (IGFBP6) were significantly negatively correlated (P<0.05). Supplementation with starter may promote rumen development by regulating the expression of genes related to rumen epithelial proliferation and nutrient generation. This study provides a basis for further exploring the mechanism of supplemental starter on rumen development.
汪晓娟, 吕凤, 庞鑫, 刘国华, 赵海碧. 早期补饲开食料对羔羊瘤胃组织转录组的影响[J]. 农业生物技术学报, 2022, 30(3): 517-527.
WANG Xiao-Juan, LYU Feng, PANG Xin, LIU Guo-Hua, ZHAO Hai-Bi. Effects of Early Supplementation with Starter on Rumen Tissue Transcriptome in Lambs (Ovis aries). 农业生物技术学报, 2022, 30(3): 517-527.
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