Abstract：Golden2-like (G2-like) transcription factors play important roles during chloroplast development, chlorophyll accumulation, photosynthesis and senescence in plants. In order to explore the role of G2-like transcription factor during grape (Vitis vinifra) development, bioinformatics method was used to identifiy G2-like family transcription factor in grape, on the basis of phylogenetic, conserved domain and synteny analysis, expression of G2-like in different organs and during rachis browning was quantified. A total of 43 G2-like (VvG1~VvG43) transcription factors uneven distributed in 17 chromosomes were identified in grape genome, and were classified into 6 groups based on phylogeny analysis with Arabidopsis thaliana. 34 pairs of synteny between A. thaliana and grape, 5 segmental duplication, 1 tandem duplication and conserved Myb DNA-binding domain were found in grape. qPCR results revealed that VvG1, VvG28, VvG43, VvG2, VvG14, VvG6, VvG24 showed positive correlation with chlorophyll content variation during rachis browning of grape, but VvG27 showed negative expression pattern. This results indicated G2-like genes played the important role in tissue development and rachis browning of grape, which provides a reference basis for further function analysis of G2-like genes.
李志谦, 邹东方, 李靖雯, 叶霞, 冯建灿. 葡萄G2-like转录因子家族的鉴定及在穗梗褐变过程中的表达分析[J]. 农业生物技术学报, 2022, 30(9): 1713-1723.
LI Zhi-Qian, ZOU Dong-Fang, LI Jing-Wen, YE Xia, FENG Jian-Can. Characterization of the G2-like Transcription Factor Family of Grape (Vitis vinifra) and Its Expression Analysis in Rachis Browning. 农业生物技术学报, 2022, 30(9): 1713-1723.
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