Mapping and Candidate Gene Analysis of Awn Inhibition Gene Hd in Wheat (Triticum aestivum)
YANG Si-Qing1, HU Le-Jia2, HU Xin2*, RONG Jun-Kang2
1. College of Forestry and Biotechnology,Zhejiang A&F University,Lin'an 311300,China; 2. College of Agriculture and Food Science,Zhejiang A&F University,Lin'an 311300,China
Abstract：Wheat (Triticum aestivum) awn,an important agronomic character of wheat controlled by many genes,plays an important role in resisting insect pests,seed reproduction and increasing wheat yield. CASL4AL is a chromosome arm substitution line (CASL) of wild emmer (T. dicoccoides,TTD) accession TTD140 in the genetic background of common wheat variety Chinese Spring (T. aestivum cv. Chinese Spring,CS),the long arm of chromosome 4A of CASL4AL is replaced by TTD140,showing long awn. In this study,a F2 population with 134 lines was constructed by using CASL4AL with long awn and the line of 442 with hook awn (derived from the cross of CASL4AL×CS,hooded awn as CS). The SSR molecular markers were used for QTL mapping for the awn length of wheat by ICIM (inclusive composite interval mapping) method. A QTL with LOD value of 30.71 was detected on the chromosome 4AS related to awn length,explaining 82.63% of the phenotypic variation. In this mapping interval,the annotation of the variants (SNP and InDel) for the CASL4AL resequencing data were conducted according to reference wheat genome,combined with the differential analysis of transcriptome sequencing,five differentially expressed genes with sense variation were obtained,among which,TraesCS4A02G078700 translates MADS-box transcription factor 1,which involved in the differentiation and proliferation of the meristem of lemma and palea,and probably involved in regulating the growth of wheat awn. This study provide reference for the screening of fine mapping materials and fine mapping of awn gene Hd (Hooded).
杨思晴, 胡乐佳, 胡鑫, 戎均康. 小麦芒抑制基因Hd的定位和候选基因分析[J]. 农业生物技术学报, 2020, 28(11): 1901-1912.
YANG Si-Qing, HU Le-Jia, HU Xin, RONG Jun-Kang. Mapping and Candidate Gene Analysis of Awn Inhibition Gene Hd in Wheat (Triticum aestivum). 农业生物技术学报, 2020, 28(11): 1901-1912.
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