Analysis of the Antennal Transcriptome and Identification of Olfaction-related Genes of Trialeurodes vaporariorum
CAI Sai-Bo1,2, YIN Chao-Kun3, ZHANG Man1,2, LIN Lian-Bing1,2, ZHANG Qi-Lin1,2*
1. Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China; 2. Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College,Kunming 650500,China; 3. School of Electrical and Information Engineering,Changzhou Institute of Technology,Changzhou 213032,China
Abstract：Trialeurodes vaporariorum is a worldwide pest with the characteristics of multi feeding and wide host range. Antennae is an important organ for insect information exchange,and its olfaction-related genes are an important molecular basis for regulating insect feeding behavior. However,to date,there are few reports about gene data of T. vaporariorum antennae,and little is known about the number and type of primary olfaction-related genes. The purpose of this study is to establish a transcriptomic database of T. vaporariorum antennae,and to investigate the number of genes encoding olfaction-related proteins. The antennal transcriptomes of adult T. vaporariorum were sequenced using Illumina HiSeq 4000 platform,and bioinformatic analyses were conducted. In this study,32 890 unigenes were obtained through assembling after transcriptome sequencing,with mean length of 1 616 bp and an N50 value of 3 059 bp,and 14 206 unigenes over 1 000 bp in size．After annotating for unigenes in gene function database,17 846 unigenes obtained functional annotation information. Sequences searched into NR database (non-redundant protein sequence database) accounted for 88.77% of all annotated unigenes,most of which was optimally hit to genes of Bemisia tabaci (87.12%) species. Annotated results in GO database showed that unigenes were divided into 54 subcategories included into 3 categories,and a lot of unigenes were annotated to be associated with metabolic process and catalytic activity. In KEGG database,5 964 unigenes were annotated in 228 metabolic pathways. Among them,most unigenes (189) were involved in metabolic pathways of ribosome. Based on further annotated information of unigenes,33 genes encoding olfaction-related proteins of T. vaporariorum including 21 genes encoding odorant binding proteins (OBPs),5 genes encoding chemosensory proteins (CSPs),2 genes encoding olfactory receptor proteins (OLRs),3 genes encoding odorant receptor proteins (ORs),1 gene encoding ionotropic receptor protein (IR) and 1 gene encoding sensory neuron membrane protein (SNMP). This study obtained the antennal transcriptomic data of adult T. vaporariorum,its characteristics were preliminarily elucidated,as well as genes encoding olfaction-related proteins were identified. This result provides a reference for further investigation on behavior regulation and new control strategies of T. vaporariorum at molecular levels,and also enriches the gene database of horticultural pests.
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