Functional Analysis of Verticillium dahliae Asp f2-like Protein (VDAL) in Fungal Pathogen Resistance and Yield Improvement in Oryza sativa
WANG Yan-Jun1,*, FU Yu-Hua2,*, ZHONG Ying-Li3, LI Hong-Liang4, REN Hai-Yang4, DONG Guo-Jun5, REN Zuo-Hua6, WANG Kai1, GAO Yuan-Hui1, GONG Zhi-Zhong1, YUAN Xiao-Hui2,**, QI Jun-Sheng1,**
1 State Key Laboratory of Plant Physiology and Biochemistry/Department of Plant Science, College of Biological Sciences, China Agricultural University, Beijing 100193, China; 2 School of Computer Science and Technology, Wuhan University of Technology, Wuhan 430070, China; 3 College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China; 4 Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, China; 5 China Rice Research Institute, Hangzhou 311400, China; 6 College of Plant Protection, Hunan Agriculture University, Changsha 410128, China
Abstract：Peptides secreted by pathogens serve as elicitors of plant defense responses. Verticillium dahliae Aspf2-like protein (VDAL) is secretory protein of V. dahliae and belongs to Aspergillus fumigatus f2-like zinc-binding protein. The VDAL146 protein in V. dahliae Vd146 strain of cotton (Gossypium spp.) was made into dry powder by engineering bacteria fermentation, and its diluent was sprayed on the leaves, which could activate the immune response of plants such as rice (Oryza sativa) and promote the growth and development of plants. In present study, VDAL146 (MT974503) was isolated and characterized, and sprayed twice before and after rice break stage. The results showed enhanced resistance to rice sheath blight, as well as improved yield by 14.5% via increasing both the number of grains per panicle and thousand grain weight. At the same time, VDAL146 did not show direct fungicidal activity. In addition, transcriptome analysis indicated that VDAL146 treatment resulted in upregulation of 322 genes involved in carbohydrate metabolism, organophosphate and glucan biosynthesis, and ATP binding. In conclusion, VDAL146 holds the potential to be developed in fungicide and biostimulant to enhance disease resistance and growth promotion in rice crop.
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