QTL Mapping for Yield-related Traits of Rice Restorer Line Luhui8258 (Oryza sativa)
CAO Ying-Jiang*, YOU Shu-Mei*, JIANG Kai-Feng, ZHANG Tao, YANG Li, YANG Qian-Hua, WAN Xian-Qi, LI Zhao-Xiang, GAO-Lei, Zheng Jia-Kui**
Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences/Luzhou Branch of National Rice Improvement Center/Key Laboratory of Southwest China Rice Biology and Genetic Breeding, Ministry of Agriculture, Deyang 618000, China
Abstract：Restorer lines is one of the important parents of three-line hybrid rice (Oryza sativa). Excavating its high-yielding genes is great important for improving the yield of hybrid rice. A recombinant inbred lines (RIL) population derived from a cross-combination of 'Luhui8258' and 'Yanghui34'. The RIL and 2 parents were grown under 3 different environments including Deyang, Suining and Luzhou of Sichuan province, and 8 yield traits including biological yield per plant (BYP), harvest index (HI), yield per plant (YP), effective panicles per plant (EP), spikelet per panicle (SP), full grain per panicle (FGP), seed setting rate (SSR, as a percentage), 1000-grain weight (TGW) were investigated. On the basis of constructing genetic linkage map consisted 184 DNA markers, a total of 62 QTLs for yield-related traits distributing on all of the 12 chromosomes were identified. Among them, 2 QTLs synergistic alleles were derived from 'Luhui8258' were detected repeatedly in 3 environments, namely, it were qTGW3 and qFGP6-1, with the contribution rate of 5.92% (Deyang), 7.79% (Suining), 19.04% (Luzhou) and 8.80% (Deyang), 10.90% (Suining), 4.96% (Luzhou), respectively. In addition, 17 yield-related QTL detected in two of these environments, included qBYP6-2, qBYP9 (QTL for BYP), qHI3-1, qHI3-2, qHI12 (for HI), qYP3-2, qYP12 (for YP), qEP2-1, qEP9-2 (for EP), qSP6-1、qSP12 (for SP), qFGP2-1, qFGP2-2, qFGP6-2, qFGP12-2 (for FGP) , qSSR2, qSSR6-1 (for SSR). Among them, 11 QTL synergistic alleles were derived from 'Luhui8258', including qHI3-1, qHI3-2, qBYP6-2, qBYP9, qYP3-2, qEP2-1, qEP9-2, qSSR2, qSSR6-1, qSP6-1, qFGP6-2, the other 6 QTL synergistic alleles were derived from 'Yanghui 34'. This research provides the basis and technical support for fine mapping, cloning and molecular marker-assisted selection of rice yield-related traits in near future breeding program.
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