Study on Enhancement and Releasing Effect of Triangular Bream (Megalobrama terminalis) in the Middle and Lower Reaches of the Qiantang River by Microsatellite Paternity Testing
1 Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture and Rural Affaris / Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 2 College of Fisheries, Nanjing Agricultural University, Wuxi 214081, China; 3 Hangzhou City Supervision and Management Chief Station of Fishery Administration, Fishing Port and Fishing Boat, Hangzhou 310008, China
Abstract：As growing quickly, big body size and delicious, triangular bream (Megalobrama terminalis) is an important economic fish, which is suggested to breed in open water in China. It is also an important catch species in Qiantang River Basin. However, M. terminalis resources have reduced recently, due to overfishing, environmental damage, water pollution, construction of water conservancy and transportation and so on. Restocking enhancement is considered as an effective method to recover the wild resources. However, there was rarely report on the effect of restocking enhancement of M. terminalis. In order to analyze the effect of restocking enhancement of M. terminalis, 12 pairs of microsatellites primers with good amplification effect were selected from 31 pairs of microsatellite primers in this study. Based on the microsatellite marker paternity test technique, genetic typing and polymorphism analysis were carried out on 301 parents from 4 breeding farms and 371 recaptured offspring individuals. The results showed that the number of alleles of microsatellite marker ranged from 15 to 68, and the expected heterozygosity was 0.726~0.969 (mean 0.863). The range of polymorphism information content was 0.693~0.967 (mean 0.849) and all 12 loci were high polymorphic (PIC>0.5). Among them, the frequency of invalid alleles of 4 microsatellite loci was greater than 0.2, which would affect the paternity analysis by software, so the data of the 4 loci were not used in following analysis. The parentage assignment for the situation of unknown sexes showed the combined non-exclusion probability of 8 loci was 99.997 5%, according to Cervus 3.0. The results showed that there were 4 recaptured individuals from offspring of brood stocks. The study suggested that the contribution of the released populations to nature resources of M. terminalis was 1.08% in 2018. This study discussed the enhancement and releasing effect of M. terminalis, which has great significance in protecting the resources and guiding the scientific breeding and releasing of M. terminalis in the Qiantang river in the future.
张敏莹, 王邢艳, 张婉平, 童奇烈, 方弟安, 周彦锋, 徐东坡. 基于微卫星亲权鉴定对钱塘江中下游三角鲂增殖放流效果的研究[J]. 农业生物技术学报, 2020, 28(7): 1260-1268.
ZHANG Min-Ying, WANG Xing-Yan, ZHANG Wan-Ping, TONG Qi-Lie, FANG Di-An, ZHOU Yan-Feng, XU Dong-Po. Study on Enhancement and Releasing Effect of Triangular Bream (Megalobrama terminalis) in the Middle and Lower Reaches of the Qiantang River by Microsatellite Paternity Testing. 农业生物技术学报, 2020, 28(7): 1260-1268.
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