Abstract：The polymorphisms of 13 microsatellite loci of 116 Gansu Alpine Merino sheep(Ovis aries) was detected using multiplex polymerase chain reaction(PCR) and DNA sequencing technology, and the paternity testing was studied combined with the parents of this record. The purpose is to supply effective microsatellite locus for germplasm resourses evaluation and paternity testing of sheep. Results demonstrated that 13 microsatellite loci altogether discovered 111 alleles. The number of alleles was most in DRB1-INTRO2(14), and the least in SRCRCP5(5). The heterozygosity was highest in ILSTS011(0.888), while the lowest was MAF214(0.500). The other 12 microsatellite loci were highly polymorphic except MAF214 which was moderate polymorphism. The mean observed heterozygosity(Ho), the expected heterozygosity(He), and the polymorphism information content (PIC) were 0.743, 0.727, and 0.689, respectively. All above results suggested that there were a relatively high genetic diversity in Gansu Alpine Merino. The calculate exclusion probability (CPE) of the 13 locus was 0.999 94 using Cervus 2.0 software. The CPE of the 11 locus which were highly polymorphic were 0.999 85, and 12 locus were 0.999 91. MAF214 had a smaller effect on CPE, and DRB1-INTRO2 had a higher effect on CPE. It could improve the accuracy requirements of paternity, make the operation more convenient, reduce costs, and be more suitable for practical production.
