联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月8日 星期五
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
甘肃高山细毛羊微卫星遗传多样性及亲子鉴定研究
李洁1,罗玉柱1,李少斌2,刘秀3,成述儒1
1. 甘肃农业大学动物科学技术学院
2. 甘肃农业大学
3. 甘肃农业大学甘肃省草食动物生物技术重点实验室
The Microsatellite Genetic Diversity and Paternity Testing of Gansu Alpine Merino Sheep(Ovis aries)
全文: PDF (271 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 利用多重PCR和DNA测序技术对116只甘肃高山细毛羊(Ovis aries)13个微卫星座位的多态性进行检测,并结合父母本记录,开展亲子鉴定研究, 以期为绵羊种质资源评价和亲子鉴定研究提供合理有效的微卫星座位。结果表明,甘肃高山细毛羊13个微卫星座位共检测到111个等位基因,在DRB1-INTRO2座位检测到的等位基因数最多(14个),SRCRCP5座位最少(5个)。ILSTS011座位观察杂合度最高(0.888),MAF214座位最低(0.500),除MAF214座位为中度多态外,其余12个座位均为高度多态,13个微卫星座位平均观察杂合度(Ho)为0.743,期望杂合度(He)为0.727,平均多态信息含量(PIC)为0.689。表明甘肃高山细毛羊遗传多样性较丰富。通过Cervus 2.0计算,13个微卫星座位累计父权排除概率(CPE)达到了0.999 94,11个具有高度多态的座位(除MAF214和OARJMP29外)CPE达到0.999 85,12个座位达到0.999 91,其中MAF214座位(中度多态)对CPE的影响较小,DRB1-INTRO2座位(高度多态)对CPE的影响较大,对亲子鉴定的贡献较大。本研究所分析的 13个微卫星座位能够有效的提高亲子鉴定要求的精确度,可使实验操作更简便,成本降低,更适用于生产实际。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
李洁
罗玉柱
李少斌
刘秀
成述儒
关键词 甘肃高山细毛羊 微卫星标记 遗传多样性 亲子鉴定    
Abstract:The polymorphisms of 13 microsatellite loci of 116 Gansu Alpine Merino sheep(Ovis aries) was detected using multiplex polymerase chain reaction(PCR) and DNA sequencing technology, and the paternity testing was studied combined with the parents of this record. The purpose is to supply effective microsatellite locus for germplasm resourses evaluation and paternity testing of sheep. Results demonstrated that 13 microsatellite loci altogether discovered 111 alleles. The number of alleles was most in DRB1-INTRO2(14), and the least in SRCRCP5(5). The heterozygosity was highest in ILSTS011(0.888), while the lowest was MAF214(0.500). The other 12 microsatellite loci were highly polymorphic except MAF214 which was moderate polymorphism. The mean observed heterozygosity(Ho), the expected heterozygosity(He), and the polymorphism information content (PIC) were 0.743, 0.727, and 0.689, respectively. All above results suggested that there were a relatively high genetic diversity in Gansu Alpine Merino. The calculate exclusion probability (CPE) of the 13 locus was 0.999 94 using Cervus 2.0 software. The CPE of the 11 locus which were highly polymorphic were 0.999 85, and 12 locus were 0.999 91. MAF214 had a smaller effect on CPE, and DRB1-INTRO2 had a higher effect on CPE. It could improve the accuracy requirements of paternity, make the operation more convenient, reduce costs, and be more suitable for practical production.
Key wordsGansu Alpine Merino    microsatellite    genetic diversity    paternity testing
收稿日期: 2012-06-15     
通讯作者: 成述儒   
引用本文:   
李洁1,罗玉柱1,李少斌2,刘秀3,成述儒1. 甘肃高山细毛羊微卫星遗传多样性及亲子鉴定研究[J]. , 2013, 21(2): 199-205.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2013/V21/I2/199
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn