Analysis of Differences in Microbial Community Structure of Eel(Anguilla japonica) Pond Before and After Pond-drying Based on High-throughput Sequencing
WANG Rui-Ning1,2, WANG Miao1, HUANG Qiu-Biao3, YI Meng-Meng1, LI Zhong-Hui1,2, LI Qing-Yong3, ZHU De-Xing3, LU Mai-Xin1,*
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture; Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China; 2 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China; 3 Fisheries Research & Extension Center of Huizhou, Huizhou 516002, China;
Abstract：Pond-drying can kill the pernicious microbe in the pond and decomposes organic matter in the sediment, which is a remediation method commonly used during the pond culture. Therefore, it is important to investigate the diversity, distribution and function of microorganisms in the pond before and after pond-drying. The present study investigated the effects of pond-drying on the aquaculture environment and microbial community structure in the water and sediment before and after pond-drying in the eel (Anguilla japonica) culture pond. The contents of ammonia nitrogen (NH4+), nitrite (NO2-), nitrate (NO3-), total nitrogen (TN), total phosphorus (TP) were measured and the changes of microbial community structure were analyzed through high-throughput sequencing. The results showed that pond-drying had significantly decrease effect on the contents of NO2-, NO3-, TN and TP in water (P<0.05) as well as the contents of TP in sediment (P<0.05). The results of high-throughput sequencing analysis showed that Proteobacteria was the main kind of bacteria in the water and sediment after pond-drying, and the abundances of Proteobacteria, Nitrospirae and Bacteroidetes were increased, while the pernicious microbe such as Cyanobacteria was decreased in the pond at the level of the phylum. At the genus level, the beneficial bacteria such as Novosphingobium, Sediminibacterium, Limnohabitans and Rhodobacter were increased, while the pernicious microbe such as Microcystis and Ellin6067 were decreased. This current study showed that pond-drying could change the microbial community structure in the water and sediment, and have a significant effect on the pond environment remediating. It provides some theoretical references for improving the environment of aquaculture pond in the future.
王瑞宁, 王淼, 黄秋标, 衣萌萌, 李忠徽, 李庆勇, 朱德兴, 卢迈新. 基于高通量测序的晒塘前后鳗鲡养殖池塘微生物群落结构差异分析[J]. 农业生物技术学报, 2020, 28(7): 1250-1259.
WANG Rui-Ning, WANG Miao, HUANG Qiu-Biao, YI Meng-Meng, LI Zhong-Hui, LI Qing-Yong, ZHU De-Xing, LU Mai-Xin. Analysis of Differences in Microbial Community Structure of Eel(Anguilla japonica) Pond Before and After Pond-drying Based on High-throughput Sequencing. 农业生物技术学报, 2020, 28(7): 1250-1259.
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