Abstract：According to the hadro-affinity principle of biotin and streptavidin, Microsatellite DNAs were isolated from Erythroculter ilishaeformis genome by combining biotin capture method and radioactive labeling hybridization. In this experiment, 613 (38.31%)positive colonies were obtained through 1400 colonies. Sequencing of 288 positive colonies confirmed that it contained 280 microsatellite loci. From these sequences,204 repeat motifs (about 72.86%) were perfect,49 repeat motifs (about 17.5%) were imperfect, 27 repeat motifs (about 9.64%) were compound. 243 pairs of primers were designed with the software Primer 3.0 .In addition, 50 pairs of primers were composed and screened. 30 pairs were used successfully to amplify special fragemnts, among which 12 pairs were polymorphism within species
