Abstract：Abstract：It is a potential valuable way using transgenic Cholrella as a bioreactor to produce alien proteins. In this paper, the vector containing the NP-1 gene under the control of the Ubiquitin promoter and two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of C. ellipsoidea via a protoplast transformation method. The transformed nrm-4 cells were screened on the medium with nitrate and G418. The transgenic lines were characterized by PCR amplification of both the NP-1 gene and NPTII genes from genomic DNA isolated from transformants. The transgenic nrm-4 was further cultured in the liquid medium with nitrate but without G418. In vitro assay results showed that the protein of the transgenic C. ellipsoidea had effective bacteria- resistance activities and it is stable without G418 selection stress. The research provided a new way to express alien genes in Chlorella, and the basis to largely produce NP-1.
