Abstract：Moso bamboo (Phyllostachys edulis) is an important economic bamboo species in China. The normal development of bamboo bud/shoot determines its yield. The MONOCULM1 (MOC1) gene plays an important role in the formation and development of axillary meristems and axillary buds. In this study, 2 MOC1 homologous genes, PheMOC1a (GenBank No. ON804247) and PheMOC1b (GenBank No. ON804248), were obtained from moso bamboo by RT-PCR. The sequence analysis showed that the ORFs of PheMOC1a and PheMOC1b were 1 530 and 1 293 bp, encoding 509 and 430 amino acid, respectively. Both polypeptide chains contain GRAS-superfamily conserved domains and belong to the GIBBERELLIN ACID INSENSITIVE (GAI), REPRESSOR of GA1 (RGA) and SCARECROW (SCR) (GRAS) gene family. Phylogenetic tree showed that PheMOC1a/b was closely related to MOC1 in rice (Oryza sativa). qRT-PCR results showed that the PheMOC1a and PheMOC1b showed obvious tissue-specific expression. PheMOC1a and PheMOC1b showed opposite expression characteristics in the bamboo bud/shoot at different developmental stages. With the development of bamboo bud/shoot, the expression of PheMOC1a decreased, while the expression of PheMOC1b increased. The trend of PheMOC1a and PheMOC1b expression was opposite in the branches at different developmental stages. The results of yeast two-hybrid and bimolecular fluorescence complementation showed that PheMOC1a and PheMOC1b not only interacted with themselves, but also interacted with each other, and interacted with 2 members of the indeterminate domain (IDD) family. These interacting proteins played biological roles in the nucleus. This study provides a reference for further analysis of the biological functions of PheMOC1a/b, and basic information for the molecular mechanism of bamboo bud/shoot development in moso bamboo.
[1] 陈骄羽. 2020. 毛竹IDD基因家族与笋芽发育的相关性研究[D]. 硕士学位论文, 浙江农林大学, 导师: 郭小勤, pp. 1-67. (Chen J Y.2020. The correlation between IDD gene family and development of shoot/bud in Phyllostachys edulis[D]. Thesis for M.S., Zhejiang A&F University, Supervisor: Guo X Q, pp. 1-67.) [2] 董丽莉, 孙化雨, 赵韩生, 等. 2014. 毛竹GRAS基因家族的全基因组分析与表达检测[C]. 第十届中国竹业学术大会论文集, 中国林学会, pp. 85-93. (Dong L L, Sun H Y, Zhao H S, et al.2014. Genme-wide analysis of GRAS gene family and gene expression detection in Phyllostachys edulis[C]. 10th China Bamboo Congress Proceedings, Chinese Society of Forestry, pp. 85-93.) [3] 何文兴, 李洪梅, 叶春江, 等. 2011. 赖草根茎分蘖相关基因LRC1的克隆及表达[J]. 中国草地学报, 33(5): 7-14. (He W X, Li H M, Ye C J, et al.2011.Cloning and expression of gene LRC1 related to rhizome tillering of Leymus secalinus[J]. Chinese Journal of Grassland, 33(5): 7-14.) [4] 李旭娟, 李纯佳, 徐超华, 等. 甘蔗MOC1基因(ScMOC1)的克隆与表达分析[J].2017. 植物遗传资源学报, 18(4): 734-746. (Li X J, Li C J, Xu C H, et al.2017. Cloning and expression analysis of the MOC1 gene (ScMOC1) in sugarcane (Saccharum officinarum)[J]. Journal of Plant Genetic Resources, 18(4): 734-746.) [5] 刘丽, 陈骄羽, 邵明侠, 等. 2021. 毛竹PheFT6和PheFT17基因对外界环境的应答及蛋白互作分析[J]. 农业生物技术学报, 29(3): 506-520. (Liu L, Chen J Y, Shao M X, et al.2021. Responses of PheFT6 and PheFT17 genes in Phyllostachys pubescens to external environment and protein interaction analysis[J]. Journal of Agricultural Biotechnology, 29(3): 506-520.) [6] 赵钟毓, 侯丹, 胡秋涛, 等. 2020. 毛竹PeNAC047基因的克隆与表达分析[J]. 农业生物技术学报, 28(1): 58-71. (Zhao Z Y, Hou D, Hu Q T, et al.2020. Cloning and expression analysis of Phyllostachys edulis PeNAC047 gene[J]. Journal of Agricultural Biotechnology, 28(1): 58-71.) [7] Bolle C, Koncz C, Chua N H.2000. PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction[J]. Genes & Development, 14(10): 1269-1278. [8] Chang W J, Liu X W, Zhang Z L.2013. Progress in molecular structure and functions of plant GRAS proteins[J]. Chinese Bulletin of Life Sciences, 25(11): 1045-1052 [9] Eloy N B, Lima M D F, Dammeet D V, et al.2011. The APC/C subunit 10 plays an essential role in cell proliferation during leaf development[J]. Plant Journal, 68(2): 351-363. [10] Fan C, Ma J, Guo Q, Li X, et al.2013. Selection of reference genes for quantitative real-time PCR in bamboo (Phyllostachys edulis)[J]. PLOS ONE, 8(2), e56573. [11] Fukazawa J, Teramura H, Murakoshi S, et al.2014. DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis[J]. Plant Cell, 26(7): 2920-2938. [12] Gamuyao R, Nagai K, Ayano M, et al.2017. Hormone distribution and transcriptome profiles in bamboo shoots provide insights on bamboo stem emergence and growth[J]. Plant & Cell Physiology, 58(4), 702-716. [13] Greb T, Clarenz O, Schafer E, et al.2003. Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation[J]. Genes & Development, 17(9): 1175-1187 [14] Hirano Y, Nakagawa M, Suyama T, et al.2017. Structure of the SHR-SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD[J]. Nature Plants, 3(3): 17010-17019. [15] Hou D, Li L, Ma T, et al.2021. The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii[J]. Horticulture Research, 8(1):133-145. [16] Li J, Smith S M, Wang B.2013. Genetic regulation of shoot architecture[J]. Annual Review of Plant Biology, 69: 437-468. [17] Li X, Qian Q, Fu Z, et al.2003. Control of tillering in rice[J]. Nature, 422(6932): 618-621 [18] Liao Z, Yu H, Duan J, et al.2019. SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice[J]. Nature Communications, 10(1): 2738-2747. [19] Livak K J, Schmittgen T.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method[J]. Methods, 25(4):402-408. [20] Marrocco K, Thomann A, Parmentier Y, et al.2009. The APC/C E3 ligase remains active in most post-mitotic Arabidopsis cells and is required for proper vasculature development and organization[J]. Development, 136(9): 1475-1485. [21] Pontes O, Neves N, Silva M, et al.2004. Chromosomal locus rearrangements are a rapid response to formation of the allotetraploid Arabidopsis suecica genome[J]. Proceedings of the National Academy of Sciences of the USA, 101(52): 18240-18245. [22] Pysh L D, Wysocka-Diller J W, Camilleri C, et al.2010. The GRAS gene family in Arabidopsis: Sequence characterization and basic expression analysis of the SCARECROW-LIKE genes[J]. Plant Journal for Cell & Molecular Biology, 18(1): 111-119. [23] Schumacher K, Schmitt T, Rossberg M, et al.1999. The LATERAL SUPPRESSOR (LS) gene of tomato encodes a new member of the VHIID protein family[J]. Proceedings of the National Academy of Sciences of the USA, 96(1): 290-295 [24] Shao G N, Lu Z F, Xiong J S, et al.2019. Tiller bud formation regulators MOC1 and MOC3 cooperatively promote tiller bud outgrowth by activating FON1 expression in rice[J]. Molecular Plant, 12(8): 1090-1102. [25] Tian C, Wan P, Sun S, et al.2004. Genome-wide analysis of the GRAS gene family in rice and Arabidopsis[J]. Plant Molecular Biology, 54(4): 519-532. [26] Ward S P, Leyser O.2004. Shoot branching[J]. Current Opinion in Plant Biology, 7(1): 73-78. [27] Zhang B, Liu X, Xu W, et al.2015. Novel function of a putative MOC1 ortholog associated with spikelet number per spike in common wheat[J]. Scientific Reports, 5(1): 12211. [28] Zhang M, Hu S, Yi F, et al.2021. Organelle visualization with multicolored fluorescent markers in bamboo[J]. Frontiers in Plant Science, 12: 658836. [29] Zhang Z, Sun X, Ma X, et al.2020. GNP6, a novel allele of MOC1, regulates panicle and tiller development in rice[J]. The Crop Journal, 9(1): 57-67. [30] Zhao J, Gao P, Li C, et al.2019. PhePEBP family genes regulated by plant hormones and drought are associated with the activation of lateral buds and seedling growth in Phyllostachys edulis[J]. Tree Physiology, 39(8): 1387-1404.
