Cloning, Expression and Interaction with AP2/ERF Transcription Factor Analysis of CAMTA1 Gene from Corchorus capsularis
ZHANG Gao-Yang1,*, DENG Jie-Lou1, XU Jian-Tang2,*, ZHANG Chao1, ZHOU Yue3, YE Shui-Feng1, WU Ying-Bao1, YANG Xin1, JIANG Lei1, HUANG Si-Qi4, LI De-Fang4
1 College of Life Sciences, Shangrao Normal University, Shangrao 334001, China; 2 College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3 Jiangxi Medical College, Shangrao 334001, China; 4 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410000, China
Abstract：Calmodulin-binding transcription factor (CAMTA) plays an important role in the regulation of plant growth and development and response to abiotic and biological stress. Jute (Corchorus capsularis) is an important commercial crop of bast fiber. In order to study the role of CAMTA in response to drought stress in jute, the 'Corchorus capsularis 179' was used as the material, primers were designed based on the sequence of CAMTA gene (CC.02G0003260) from the jute genome. The CAMTA gene was obtained by RT-PCR and named as CAMTA1 (OK415793). Sequence analysis showed that the CAMTA1 gene contained 13 exons and 12 introns, Its open reading frame was 3 051bp and encoded a protein containing 1 016 amino acids, which had the CG-1 domain, TIG domain, ANK repeat sequence and IQ motif of CAMTA proteins. Protein sequence alignment indicated that CAMTA1 had high homology with the protein of Malvaceae plants. qPCR analysis showed that CAMTA1 gene was expressed in root, stem, leaf and pericarp. The expression of CAMTA1 gene in leaves reached the highest at 6.0 h after drought stress induction. Fluorescence enzyme protein complementation assay suggested that CaMTA1 interacted with another stress-responsive transcription factor AP2/ERF to regulate plant growth, development and respond to both abiotic and biological stresses.These results provide a theoretical basis for further elucidating the molecular mechanism of CAMTA1 gene involved in plant drought response.
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