Identification of Hsp70 Gene Family in Fusarium verticillioides & F. graminearum and Their Expression Patterns at Different Temperatures
CHEN Yue1,2, LI Hai-Xiao2,3, LIU Zheng4, LIU Ning2,3, SUN Man-Li2,3, CAO Zhi-Yan2,3,*, DONG Jin-Gao2,3,*
1 College of Life Science, Hebei Agricultural University, Baoding 071000, China; 2 Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China; 3 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China; 4 Academy of Educational Sciences, Baoding 071066, China
Abstract：Fusarium verticillioides and F. graminearum are the main pathogens that cause stalk rot, ear rot and other diseases in maize (Zea mays), which can reduce the yield and quality of maize, and affect mechanized harvesting. At the same time, mycotoxins produced by pathogens can seriously threaten the health of human and livestock. Heat shock proteins (HSP) can be expressed largely when the organism is under environmental stress to prevent protein denatured and improve the organism's resistance to various adversities. Currently, Hsp70 is the most conservative and widely studied type. In order to investigate whether the growth of F. verticillioides and F. graminearum were regulated by temperature, F. verticillioides and F. graminearum were inoculated into the culture medium with the same growth period, and place them in the incubator at 20, 25 and 30 ℃ respectively. It was found that F. verticillioides grew rapidly at 30 ℃, with dense hyphae, while F. graminearum grew better at 20 ℃. In order to further clarify whether HSP70 gene family is involved in regulating the growth and development of F. verticillioides and F. graminearum under adverse temperature, nine HSP70 genes were identified in F. verticillioides genome and 6 HSP70 genes were identified in F. graminearum genome through genome-wide search. The relative expression levels of Hsp70 gene family in F. verticillioides and F. graminearum cultured at 20, 25 and 30 ℃ were detected by qPCR. The results showed that the expression levels of FvHsp70-2, FvHsp70-4, FvHsp70-5 and FvHsp70-6 in the F. verticillioides Hsp70 gene family were significantly increased at 20 ℃ (P<0.05), especially the gene FvHsp70-2, which was about 4.5 times higher at 20 ℃ than at 30 ℃. In F. graminearum, while the expression levels of FgHsp70-6 closely related to FvHsp70-6, FgHsp70-2 closely related to FvHsp70-2, FgHsp70-4 closely related to FvHsp70-4, and FgHsp70-3 closely related to FvHsp70-5 were significantly increased at 30 ℃ (P<0.05). These results indicated that Hsp70 genes are responsive to adverse temperature in F. verticillioides and F. graminearum, and the expression patterns of Hsp70 family genes established at different temperatures can provide a theoretical basis for analyzing the molecular mechanism of Hsp70 influencing temperature change to regulate the growth of F. verticillioides and F. graminearum.
陈悦, 李海笑, 刘峥, 刘宁, 孙蔓莉, 曹志艳, 董金皋. 拟轮枝镰孢和禾谷镰孢Hsp70基因家族鉴定及其在不同温度下的表达模式[J]. 农业生物技术学报, 2022, 30(12): 2407-2416.
CHEN Yue, LI Hai-Xiao, LIU Zheng, LIU Ning, SUN Man-Li, CAO Zhi-Yan, DONG Jin-Gao. Identification of Hsp70 Gene Family in Fusarium verticillioides & F. graminearum and Their Expression Patterns at Different Temperatures. 农业生物技术学报, 2022, 30(12): 2407-2416.
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