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2025年8月11日 星期一
农业生物技术学报  2022, Vol. 30 Issue (11): 2061-2076    DOI: 10.3969/j.issn.1674-7968.2022.11.001
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
大豆醛酮还原酶超家族的鉴定及表达分析
厉苏宁, 赵现伟, 孙丽萍, 赵朝森, 王瑞珍, 郭兵福*
江西省农业科学院 作物研究所/国家油料改良中心南昌分中心/江西省油料作物生物学重点实验室,南昌 330200
Identification and Expression Analysis of AKR Superfamily in Soybean (Glycine max)
LI Su-Ning, ZHAO Xian-Wei, SUN Li-Ping, ZHAO Chao-Sen, WANG Rui-Zhen, GUO Bing-Fu*
Crops Research Institute/Nanchang Branch of National Center of Oilcrops Improvement/Jiangxi Province Key Laboratory of Oilcrops Biology, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
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摘要 醛酮还原酶(aldo keto reductases, AKRs)广泛分布于动植物中,与外源性和内源性毒素代谢有关,包括应激刺激产生的有毒物质,在类固醇、糖和其他羰基代谢中发挥着重要作用。目前已经在拟南芥(Arabidopsis thaliana)和水稻(Oryza sativa)等植物中报道了一些参与逆境胁迫的AKR基因,但大豆中相关研究仍较少。本研究采用生物信息学方法和qPCR技术对大豆(Glycine max) AKR家族成员进行全基因组鉴定及表达特征分析。在大豆基因组中共鉴定到44个醛酮还原酶GmAKR基因,其编码的蛋白均具有Aldo-ket-red结构域。系统进化分析显示,GmAKR基因可以聚为5个家族,不均匀地分布在大豆的16条染色体上。GmAKR各成员启动子区域均存在数量不等的光响应、激素和逆境响应顺式作用元件,表明该家族基因可能参与大豆的多种生长发育调节过程。基因表达分析结果验证了GmAKR基因在不同组织中的表达模式,与Phytozome数据库收入结果基本一致。本研究为深入揭示大豆AKR家族基因的生物学功能提供了参考。
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厉苏宁
赵现伟
孙丽萍
赵朝森
王瑞珍
郭兵福
关键词 大豆醛酮还原酶(AKRs)生物信息学分析表达模式    
Abstract:Aldo keto reductases (AKRs) are widely distributed in plants and animals and play an important role in the metabolism of steroids, sugars, and other carbonyls. It is associated with the metabolism of exogenous and endogenous toxins, including toxic substances produced by stimulation. At present, some AKR genes involved in stress had been reported in plants such as Arabidopsis thaliana and rice (Oryza sativa), but the research of AKRs in soybean (Glycine max) are still scarce. In this study, bioinformatics methods and qPCR were used to identify the AKR family members in soybean on whole genome and analyze its expression characteristics. The results showed that 44 GmAKR genes in soybean genome, and the proteins encoded by GmAKRs all had the Aldo-ket-red domain. Phylogenetic analysis showed that GmAKRs could be clustered into 5 families, which distributed unevenly on 16 chromosomes. The promoter regions of GmAKRs had different numbers of hormone or stress-responsive cis-acting elements, suggested that GmAKRs may be involved in various growth and development regulation processes of plants. Expression pattern analysis showed that GmAKRs had different tissue expression characteristics, and was basically consistent with the Phytozome database. These results provide a reference for further revealing the biological functions of AKR family in soybean.
Key wordsSoybean    Aldo keto reductases (AKRs)    Bioinformatics analysis    Expression pattern
收稿日期: 2021-12-22     
ZTFLH:  S529  
基金资助:江西省青年科学基金(20192ACBL21025); 江西省引进培养创新创业高层次人才“千人计划”(jxsq2019201073); 江西现代农业科研协同创新专项(JXXTCXBSJJ202201; JXXTCXQN201909)
通讯作者: * gbfhq@163.com   
引用本文:   
厉苏宁, 赵现伟, 孙丽萍, 赵朝森, 王瑞珍, 郭兵福. 大豆醛酮还原酶超家族的鉴定及表达分析[J]. 农业生物技术学报, 2022, 30(11): 2061-2076.
LI Su-Ning, ZHAO Xian-Wei, SUN Li-Ping, ZHAO Chao-Sen, WANG Rui-Zhen, GUO Bing-Fu. Identification and Expression Analysis of AKR Superfamily in Soybean (Glycine max). 农业生物技术学报, 2022, 30(11): 2061-2076.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.11.001     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I11/2061
 
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