Construction of GFP-labeled Transformant of Hypovirulent Strain FC136-2A in Fusarium pseudograminearum
WANG Zhi-Fang1, LI Ke1, LIU Dong-Wei1, XIE Yuan1, DAI Jun-Li1, WANG Ke1, GAO Fei1, ZHANG Xiao-Ting1,*, LI Hong-Lian1,2,*
1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 2 National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450046, China
Abstract：Wheat crown rot is an important soil-borne disease occurring widely in most wheat (Triticum aestivum) growing areas in the world. Fusarium pseudograminearum is the dominant causal agent of wheat crown rot. In F. pseudograminearum strain FC136-2A, mycovirus Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1) was found to cause the hypovirulence of FC136-2A on wheat. To explore the mechanism of hypovirulence caused by FpgMBV1, a pCAM-GFP-hyg construct was transformed into F. pseudograminearum strain FC136-2A by Agrobacterium tumefaciens-mediated genetic transformation technology. Molecular identification, fluorescent observation, biological characterization and pathogenicity test of the transformants were also conducted. Results showed that the GFP-labeled transformant FC136-2A-GFP had no biological differences compared to FC136-2A in growth rate and sporulation capacity. Besides, FpgMBV1 was still in the transformant FC136-2A-GFP and the pathogenicity of FC136-2A-GFP was low on wheat. The present study obtained GFP-labeled transformant FC136-2A-GFP of F. pseudograminearum, which could be used to observe the infection and pathogenic process of hypovirulence strain FC136-2A on wheat, and provide basic information for further study on the mechanism of hypovirulence effect of mycovirus FpgMBV1 on F. pseudograminearum.
王志芳, 李轲, 刘东伟, 谢源, 代君丽, 王珂, 高飞, 张晓婷, 李洪连. 假禾谷镰孢菌弱毒菌株FC136-2A的GFP标记转化子构建[J]. 农业生物技术学报, 2021, 29(7): 1435-1442.
WANG Zhi-Fang, LI Ke, LIU Dong-Wei, XIE Yuan, DAI Jun-Li, WANG Ke, GAO Fei, ZHANG Xiao-Ting, LI Hong-Lian. Construction of GFP-labeled Transformant of Hypovirulent Strain FC136-2A in Fusarium pseudograminearum. 农业生物技术学报, 2021, 29(7): 1435-1442.
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