Genome-wide Identification and Expression Analysis Under Low-temperature Stress of Histone Gene Family in Eggplant (Solanum melongena)
LIN Hui1, HUANG Jian-Du2, CHEN Ji-Bing2, WANG Yi-Kui3, ZHU Hai-Sheng1,*, WEN Qing-Fang1,*
1 Fujian Key Laboratory of Vegetable Genetics and Breeding, Fujian Engineering Research Center for Vegetables, Crops Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350013, China; 2 Fuzhou Institute of Vegetable Science, Fuzhou 350111, China; 3 Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Abstract：Histones are essential components of chromatin and play an important role in developmental regulation, and resistance to stress. In order to explore the characteristics under cold stress in eggplant (Solanum melongena), in this study, the genome-wide identification of Histone genes and bioinformatics analysis were carried out in eggplant, the transcriptome data were mined to analyze the expression pattern of SmHistones in 2 different resistant eggplant (epl008 and eplck) leaves under low temperature stress. The results showed that a total of 40 SmHistones genes were identified in eggplant, which were unevenly distributed on 9 chromosomes. The members of SmHistone gene family were classified into 5 subgroups (H1, H2A, H2B, H3 and H4). Members of the same groups had similar physical and chemical properties, gene/protein structure, and conserved motifs. Intraspecific collinearity analysis showed that there was a collinear relationship among 6 pairs of SmHistones. The results of promoter elements analysis indicated that a large number of abiotic stress response cis-acting elements, such as low temperature responsive elements and drought responsive elements existed in promoter sequences of Smhistones. Transcriptome and qRT-PCR analysis revealed that 36 Smhistones were differentially expressed under low temperature stress and were significantly upregulated in eggplant epl008. The induced expression of SmHistone3 under low temperature stress was significant, revealing that it may be closely related to the cold metabolic response. This study provides a theoretical basis in breeding of eggplant with high tolerance to low temperature stress.
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