Cloning, Expression and Preliminary Functional Analysis of LibHLH75 Gene from Lavender (Lavandula×intermedia)
Maierziyaguli•ABULAIKEMU, ZHANG Jia-Jun, YAN Zhong-Jian, LIAO Yan, SHI Mei-Lan, LI Xue-Long, WANG Ai-Fan*, SU Xiu-Juan*
College of Agriculture/Xinjiang Key Lab of Crop Improvement & Germplasm Enhancement/Lavender Research Institute, Xinjiang Agricultural University, Urumqi 830052, China
Abstract：Lavender (Lavandula) is an important aromatic crop, and the differences in the quality of its essential oil are mainly caused by variations in the content of terpenoids, such as linalool and linalyl acetate. Studying the relevant genes that may be involved in regulating the biosynthesis of essential oils in lavender can provide genetic resources for breeding excellent lavender germplasm. The basic Helix-Loop-Helix (bHLH) transcription factor family plays a crucial role in plant growth, development, and secondary metabolism. In this study, the lavender LibHLH75 gene (GenBank No. OR019688) was cloned from the 'Zahua' of lavender (Lavandula×intermedia）, with an open reading frame of 831 bp, encoding 276 amino acids, which was closest to the bHLH75-like protein from Salvia splendens and bHLH75 protein from S. hispanica. qRT-PCR analysis showed that the LibHLH75 gene was expressed in different tissues of lavender and at different flowering stages of the corolla, with the highest expression level at the calyx and bud stages. The results of subcellular localization and transcriptional activation activity identification indicated that LibHLH75 encoded protein was localized in the nucleus, and had transcriptional activation activity. This study preliminary analyzed the function of LibHLH75, which provides a theoretical basis for further revealing the molecular regulatory mechanism of bHLH transcription factors in the biosynthesis of lavender terpenoids.
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