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2025年4月6日 星期日
农业生物技术学报  2024, Vol. 32 Issue (1): 70-79    DOI: 10.3969/j.issn.1674-7968.2024.01.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
过表达F-box基因BoFBX117对甘蓝低温耐受性的影响
罗玉霞, 许涛, 戴倩, 陈瑶, 宋江华*
安徽农业大学 园艺学院,合肥 230036
Effect of Overexpression of F-box Gene BoFBX117 on Low-temperature Tolerance of Cabbage (Brassica oleracea var. capitata)
LUO Yu-Xia, XU Tao, DAI Qian, CHEN Yao, SONG Jiang-Hua*
School of Horticulture, Anhui Agricultural University, Hefei 230036, China
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摘要 F-box基因在植物非生物胁迫响应过程中发挥重要作用。为了研究F-box基因BoFBX117(GenBank No. XM_013731217.1)在甘蓝(Brassica oleracea var. capitata)响应低温胁迫中的功能,本研究构建了BoFBX117过表达载体,通过农杆菌介导法获得过表达BoFBX117甘蓝(OE-BoFBX117)植株。在4、0、-2和-4 ℃低温胁迫24 h后,通过观察表型发现过表达BoFBX117甘蓝叶片黄化萎蔫程度均小于非转基因甘蓝(WT);经过7 d恢复期,发现过表达BoFBX117甘蓝恢复正常生长能力更强。qPCR结果表明,低温胁迫下转基因植株中BoFBX117的表达量显著升高(P<0.01),是WT植株的1.87~4.80倍。0 ℃低温胁迫时,转基因植株的超氧化物歧化酶(superoxide dismutase, SOD)、过氧化物酶(peroxidase, POD)和过氧化氢酶(Catalase, CAT)活性最高,分别比WT植株提高80%、16%和99%。低温胁迫下,丙二醛(malondialdehyde, MDA)含量不断积累,-4 ℃时转基因植株的丙二醛含量为0.62 μmol/g FW,比WT降低53.38%。在正常条件和低温胁迫下,转基因植株的脯氨酸(proline)和可溶性糖(soluble sugar)含量均显著高于WT植株(P<0.05)。结果表明,BoFBX117在低温胁迫应答过程中发挥正调控作用,过表达BoFBX117提高了转基因甘蓝的低温耐受性,本研究为甘蓝耐低温品种选育提供支持。
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罗玉霞
许涛
戴倩
陈瑶
宋江华
关键词 甘蓝F-box基因BoFBX117低温胁迫    
Abstract:F-box gene plays an important role in plant abiotic stress response. In order to study the function of F-box gene BoFBX117 (GenBank No. XM_013731217.1) in cabbage (Brassica oleracea var. capitata) response to low-temperature stress, in this study, the BoFBX117 over-expression vector was constructed, and the over-expression of BoFBX117 cabbage (OE-BoFBX117) plants were obtained by Agrobacterium tumefaciens mediated method. After 24 h of low-temperature stress at 4, 0, -2 and -4 ℃, the phenotype of the over-expression of BoFBX117 cabbage showed that the degree of leaf yellowing wilting was less than that of non-transgenic cabbage (WT). After 7 d of recovery, it was found that the over-expression of BoFBX117 cabbage had stronger ability to return to normal growth. The results of qPCR showed that the expression of BoFBX117 in transgenic plants was significantly increased under low-temperature stress (P<0.01), which was 1.87~4.80 times that of WT plants. Under low-temperature stress at 0 ℃, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of transgenic plants were the highest, which were 80%, 16% and 99% higher than those of WT plants respectively. Under low-temperature stress, the content of malondialdehyde (MDA) continuously accumulated. At -4 ℃, the MDA content of transgenic plants was 0.62 μmol/g FW, 53.38% lower than WT. Under normal conditions and low-temperature stress, the proline and soluble sugar contents of transgenic plants were significantly higher than those of WT plants (P<0.05). The results showed that BoFBX117 might play a positive role in the response to low-temperature stress, and over-expression of BoFBX117 in cabbage could improve the low-temperature tolerance of cabbage. This study provides support for the breeding of low-temperature tolerance varieties of cabbage.
Key wordsBrassica oleracea var. capitata    F-box gene    BoFBX117    Low-temperature stress
收稿日期: 2022-12-15     
ZTFLH:  S635.1  
基金资助:国家自然科学基金(32272728; 31872108)
通讯作者: *jhsong@ahau.edu.cn   
引用本文:   
罗玉霞, 许涛, 戴倩, 陈瑶, 宋江华. 过表达F-box基因BoFBX117对甘蓝低温耐受性的影响[J]. 农业生物技术学报, 2024, 32(1): 70-79.
LUO Yu-Xia, XU Tao, DAI Qian, CHEN Yao, SONG Jiang-Hua. Effect of Overexpression of F-box Gene BoFBX117 on Low-temperature Tolerance of Cabbage (Brassica oleracea var. capitata). 农业生物技术学报, 2024, 32(1): 70-79.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.01.007     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I1/70
 
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