Epigenetic Mechanisms of Reprogramming of Animal Somatic Cell Nuclear Transfer Embryos
JI Yu-Chen1, LI Yi-Han2, WANG Mei-Juan1, ZHENG Rui-Zhi1, YU Tong1, SU Jian-Min1,*
1 Key Laboratory of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; 2 Key Laboratory of Animal Biotechnology, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
Abstract：Somatic cell nuclear transfer (SCNT) technology can be used to expand the production of top livestock and gene editing animals. So far, humans have successfully cloned more than 20 kinds of animals, but the efficiency of cloned animals obtained through somatic cell nuclear transfer technology is relatively low. Most somatic cell nuclear transfer embryos die before implantation. After nuclear transfer, the donor nucleus will undergo a series of reprogramming events to obtain the totipotency of embryonic development. The abnormal reprogramming of somatic cell nuclear transfer embryos is the main reason leading to the low efficiency of somatic cell nuclear transfer. In this paper, the abnormal reprogramming mechanisms of somatic cell nuclear transfer embryos were analyzed, such as chromatin remodeling, DNA methylation, variations and modifications of histones, sperm small RNA, etc., so as to provide reference for further research on the reprogramming of somatic cell nuclear transfer embryos.
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