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2025年8月5日 星期二
农业生物技术学报  2024, Vol. 32 Issue (1): 107-114    DOI: 10.3969/j.issn.1674-7968.2024.01.010
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
转录因子NAC62在工业大麻中的克隆、生信分析及其干旱胁迫响应分析
杨宇蕾, 张涵雪, 王珊珊, 汤开磊, 杜光辉*
云南大学 农学院,昆明 650500
Cloning, Bioinformatics Analysis of Transcription Factor NAC62 in Industrial Hemp (Cannabis sativa) and Its Response Analysis to Drought Stress
YANG Yu-Lei, ZHANG Han-Xue, WANG Shan-Shan, TANG Kai-Lei, DU Guang-Hui*
School of Agriculture, Yunnan University, Kunming 650500, China
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摘要 工业大麻(Cannabis sativa)是一种重要经济作物,其种子萌发阶段易遭受干旱胁迫,最终对生长和产量造成不利影响。NAC基因是植物中特有的一类转录因子,在植物应对非生物胁迫方面起着重要作用。本研究基于前期的转录组数据,利用RT-PCR方法,从工业大麻品种'云麻1号'中分离克隆出了1个NAC类基因,命名为CsNAC62 (GenBank No. XM_030652694)。序列分析发现,CsNAC62基因CDS大小为1 260 bp,编码419个氨基酸,蛋白质分子量48.32 kD,等电点为8.24。CsNAC62蛋白的N端含有150~160个氨基酸组成的NAC结构域,该结构域由5个亚结构域组成,是NAM/NAC类基因家族典型的保守结构域。蛋白二级结构预测表明,CsNAC62由25.19%的α-螺旋、17.29%的延伸链和57.52%的不规则卷曲组成。系统进化分析表明,工业大麻CsNAC62与桃树(Prunus persica) NAC3的关系最近。'云麻1号'萌发种子中的CsNAC62基因随着干旱胁迫处理(PEG-6000模拟)时间的延长,其表达量也逐步增加,萌发7 d时表达量达到最大。而在正常萌发的种子(对照)中,CsNAC62的表达量则上升缓慢,表明CsNAC62基因受干旱诱导表达。本研究初步揭示了CsNAC62基因在PEG模拟干旱胁迫下的表达模式,为进一步探究其参与工业大麻干旱胁迫响应的分子机制提供参考。
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杨宇蕾
张涵雪
王珊珊
汤开磊
杜光辉
关键词 工业大麻NAC转录因子干旱胁迫生物信息学分析表达模式    
Abstract:Industrial hemp (Cannabis sativa) is an important economic crop, and its seed germination period is susceptible to drought stress, which ultimately has adverse effects on the growth and yield of hemp. NAC gene is a unique type of transcription factor in plants, which plays an essential role in plant response to abiotic stress. Based on the previous transcriptome data, a NAC gene from industrial hemp 'Yunma 1' was cloned by RT-PCR in this study, and named as CsNAC62 (GenBank No. XM_030652694). The results of sequence analysis found that the coding sequence of CsNAC62 gene contains 1 260 bp, encoding 419 amino acids, its protein molecular weight is 48.32 kD, and the isoelectric point is 8.24. The N-terminal of CsNAC62 contains a NAC domain composed of 150~160 amino acids. This domain is composed of 5 subdomains, which is a typical conservative domain of the NAM/NAC gene family. Phylogenetic analysis showed that CsNAC62 was closely related to the NAC3 protein of peach (Prunus persica) in all the tested species. The gene expression level of CsNAC62 in the germinated seeds of industrial hemp 'Yunma 1' gradually increased with the prolongation of the drought stress treatment (PEG-6000 simulation), and the expression level reached to the maximumon the 7 d of germinated seeds. In normal seed germination (control), the expression level of CsNAC62 increased slowly. These results indicated that the gene expression of CsNAC62 was induced by drought. This study preliminarily explored the expression mode of the CsNAC62 under PEG simulated drought stress, and provides a reference for further exploring its molecular mechanism of participating in the response to industrial cannabis drought stress.
Key wordsCannabis sativa    NAC transcription factor    Drought stress    Bioinformatics analysis    Expression pattern
收稿日期: 2023-04-19     
ZTFLH:  S563.3  
基金资助:国家自然科学基金(32160514); 国家麻类产业技术体系建设专项(CARS-16-E15)
通讯作者: *dgh2012@ynu.edu.cn   
引用本文:   
杨宇蕾, 张涵雪, 王珊珊, 汤开磊, 杜光辉. 转录因子NAC62在工业大麻中的克隆、生信分析及其干旱胁迫响应分析[J]. 农业生物技术学报, 2024, 32(1): 107-114.
YANG Yu-Lei, ZHANG Han-Xue, WANG Shan-Shan, TANG Kai-Lei, DU Guang-Hui. Cloning, Bioinformatics Analysis of Transcription Factor NAC62 in Industrial Hemp (Cannabis sativa) and Its Response Analysis to Drought Stress. 农业生物技术学报, 2024, 32(1): 107-114.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.01.010     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I1/107
 
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