Verification and Analysis of Cold Resistance of CcCBFc Gene from Cinnamomum camphora
WANG Jian-Ge1,2, ZHOU Chan1, LIU Yi-Lang1, DU Li1,*
1 School of Life Science and Technology, Nanyang Normal University, Nanyang 473000, China; 2 College of Landscape Architecture and Art, Henan Agricultural University College of Landscape Architecture and Art, Zhengzhou 450002, China
Abstract：CBF (CRT/DRE binding factor) gene plays an important role in ennhancing the ability of plants to resist low temperature stress. In order to verify the cold-resistance function of CcCBFc gene from Cinnamomum camphora, the mechanism of the cold-resistant effect of the CcCBFc in Arabidopsis thaliana was revealed, and the biological function of this gene in Arabidopsis against cold stress was presumed. In the early stage of this laboratory, 4 CcCBFs (CcCBFa, CcCBFb, CcCBFc, CcCBFd) were cloned, and the CcCBFc (GenBank No. KP336741) was initially verified to improve the drought resistance, salt resistance and cold resistance of the transgenic tobacco. This study was based on this, which used transgenic CcCBFc Arabidopsis and wild-type as materials to detect antioxidant enzyme activity under low temperature stress (4 ℃). Antifreeze effect of transgenic CcCBFc Arabidopsis was detected by -4 ℃ simulated freezing injury. qRT-PCR was used to detect the expression level of antioxidant gene of transgenic CcCBFc Arabidopsis at 6 d after low temperature stress, and sequenced transgenic CcCBFc Arabidopsis and wild type at transcriptional level under low temperature stress. The results showed that CcCBFc could increase the peroxidase and catalase activities of transgenic Arabidopsis, and had a positive effect on the antifreeze of transgenic Arabidopsis, and could promote the expression of antioxidant genes in transgenic Arabidopsis. Transcriptome sequencing results of the wild-type and transgenic CcCBFc Arabidopsis indicated that the function of the exogenous CcCBFc gene was related to the metabolic process, catalytic activity and binding activity, and was involved in the regulation of the endoplasmic reticulum. The synthesis of proteins and plant hormones enhanced the cold tolerance of Arabidopsis. This study provides a reference for further revealing the function of CcCBFc in the response mechanism of low temperature stress.
王建格, 周婵, 刘译朗, 杜丽. 香樟CcCBFc基因抗寒功能验证及分析[J]. 农业生物技术学报, 2021, 29(2): 268-278.
WANG Jian-Ge, ZHOU Chan, LIU Yi-Lang, DU Li. Verification and Analysis of Cold Resistance of CcCBFc Gene from Cinnamomum camphora. 农业生物技术学报, 2021, 29(2): 268-278.
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