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2025年4月4日 星期五
农业生物技术学报  2022, Vol. 30 Issue (12): 2376-2384    DOI: 10.3969/j.issn.1674-7968.2022.12.010
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
ARL15基因敲除小鼠模型建立和表型分析
薛明明1, 王克君2, 产舒恒1, 雒亚彪1, 方美英1,*
1 中国农业大学 动物科学技术学院 北京 100193;
2 河南农业大学 动物科技学院,郑州 450046
Establishment and Phenotypic Analysis of ARL15 Knockout Mice (Mus musculus) Model
XUE Ming-Ming1, WANG Ke-Jun2, CHAN Shu-Heng1, LUO Ya-Biao1, FANG Mei-Ying1,*
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2 College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
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摘要 大量基因组关联分析研究发现ADP核糖基化因子样15 (ADP-ribosylation factor-like 15, ARL15)基因变异与能量代谢相关疾病显著关联。为深入研究该基因生物学功能,本研究利用CRISPR/Cas9技术首次构建了ARL15基因敲除小鼠(Mus musculus)模型,并对其表型进行了分析。一代测序结果显示,敲除了小鼠ARL15基因2号外显子在内的1 841 bp;与野生型小鼠相比,ARL15-/-小鼠ARL15基因和蛋白表达水平均显著降低(P<0.05);在胚胎期16.5 d,ARL15+/+ARL15+/-ARL15-/- 3种基因型小鼠胚胎的存活比例符合孟德尔遗传定律;ARL15-/-小鼠在出生后0.5 d死亡,且体重较轻(P<0.05);qPCR结果表明,小鼠ARL15基因缺失分别抑制了脂肪和肠道组织中脂联素(adiponectin, C1Q and collagen domain containing, Adipoq)和二酰基甘油O-酰基转移酶2 (diacylglycerol O-acyltransferase 2, DGAT2)基因的表达(P<0.05)。表明该基因对小鼠出生后早期存活和机体脂质代谢具有重要作用。本研究成功建立了ARL15基因敲除小鼠模型,可用于ARL15基因功能的深入研究。
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薛明明
王克君
产舒恒
雒亚彪
方美英
关键词 ADP-核糖基化因子样15 (ARL15)基因敲除小鼠模型表型分析    
Abstract:A massive number of genome wide association studies have found that ADP-ribosylation factor-like 15 (ARL15) variation is significantly associated with energy metabolism-related diseases. To investigate the biological function of this gene, CRISPR/Cas9 technique was used to construct ARL15 knockout mice (Mus musculus) model for the first time, and its phenotype was analyzed. First-generation sequencing results showed that 1 841 bp of ARL15 was knocked out, including exon 2. Compared with wild-type mice, the mRNA and protein expression levels of ARL15 were significantly decreased in ARL15-/- knockout mice (P<0.05). The embryo survival ratio of ARL15+/+, ARL15+/- and ARL15-/- mice on embryo 16.5 d was present at Mendelian Genetics; ARL15-/- mice had lower body weight on postnatal 0.5 d (P<0.05), and died shortly. qPCR showed that deletion of ARL15 inhibited adiponectin, C1Q and collagen domain containing (Adipoq) and diacylglycerol O-acyltransferase 2 (DGAT2) mRNA expression levels in fat and intestinal tissues of mice, respectively (P<0.05). In conclusion, ARL15 plays an important role in the early postnatal survival and lipid metabolism in mice. The ARL15 knockout mouse model has been successfully established, which can be used to further study the function of ARL15.
Key wordsADP-ribosylation factor-like 15 (ARL15)    Knockout mice model    Phenotypic analysis
收稿日期: 2021-12-31     
ZTFLH:  S185  
基金资助:国家自然科学基金(32072700; 32002142); 三亚崖州湾科技城管理局2020年度科技计划项目(SKJC-2020-02-007); 财政部和农业农村部:国家现代农业产业技术体系(CARS-36); 中国农业大学人才培养和发展支持项目
通讯作者: * meiying@cau.edu.cn   
引用本文:   
薛明明, 王克君, 产舒恒, 雒亚彪, 方美英. ARL15基因敲除小鼠模型建立和表型分析[J]. 农业生物技术学报, 2022, 30(12): 2376-2384.
XUE Ming-Ming, WANG Ke-Jun, CHAN Shu-Heng, LUO Ya-Biao, FANG Mei-Ying. Establishment and Phenotypic Analysis of ARL15 Knockout Mice (Mus musculus) Model. 农业生物技术学报, 2022, 30(12): 2376-2384.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2022.12.010     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2022/V30/I12/2376
 
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