Abstract：Chinese cabbage (Brassica rapa ssp. pekinensis) is native to China and belongs to the crucifer family Brassica genus biennial plant. It is a vegetable crop widely cultivated in our country. In this study, a genetically stable yellow cotyledon pure line (19YC-2) and normal green cotyledon near isogenic line (19GC-2) and the F1, F2 populations by hybridizing with 19YC-2 and 19GC-2 were used as materials, The genetic analysis and gene mapping of yellow cotyledon mutant in Chinese cabbage were carried out. Phenotypic observation showed that the cotyledon phenotype of F1 plants was normal green as 19GC-2, and the number of green cotyledon plants to yellow cotyledon plants in F2 population was conformed to 3∶1 proportion, which indicated that yellow cotyledon mutation was a quality trait controlled by one single recessive nuclear gene. The parental polymorphic SSR primers were further screened by bulked segregant analysis (BSA) method and the gene mapping of yellow cotyledon was made. The yellow cotyledon gene was located on the A06 linkage group of Chinese cabbage with two nearest flanking markers SSR280-23 and SSR280-42, their genetic distances are 0.04 cM and 0.96 cM, respectively, and their interval physical distance was 83.9 kb. Through genetic analysis and gene mapping of the yellow cotyledon mutants of Chinese cabbage, this study lays a foundation for the cloning and molecular mechanism of Brassica rapa yellow cotyledon (Bryc).
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