Abstract：The Pin-formed acicular protein (PIN), one of the plant auxin transporters, affects the quality of cotton (Gossypium spp.) fibers. Understanding the expression characteristics of GbPIN1a (GenBank No. KAB2059844.1) in sea-island cotton (G. barbadense) fiber can provide a reference for exploring the mechanism of fiber quality development. In this study, the gene was cloned by PCR technology, and the bioinformatics analysis was carried out; A transient expression vector containing GFP was constructed and cell localization analysis was carried out. The results showed that the length of the coding region of GbPIN1a was 1 758 bp, and 585 amino acids were encoded; two conserved domains including Mem trans were contained in amino acid sequence; GbPIN1a gene showed a high expression trend at 15 and 20 DPA (days post anthesis) of fiber development and was significantly negatively correlated with auxin content at the same time period (P<0.05); The expression level in roots and stems was significantly higher than that in leaves (P<0.05), with tissue specificity; The expression level fluctuated when stimulated by exogenous auxin; The expressed product was localized on the cell membrane. The results of this study preliminarily indicate that the GbPIN1a gene may be involved in the regulation of cotton fiber development, provide basic data for future research on PIN1a genes.
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