Joint Multi-generations Genetic Analysis on Chilling Tolerance of Melon (Cucumis melo) Seedlings
XU Xiao-Jun1, 2, LIANG Chang-Zhi1, LIU Hai-Ying1, ZHANG Gui-Lan2, LIU Ke-Ke1, YANG Lu-Ming1, HU Jian-Bin1, *
1 Henan Key Laboratory of Fruit and Cucurbit Biology/College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 2 Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, Zhengzhou 450009, China
Abstract：Chilling tolerance is an important reflection on the ability of plant to adopt to low-temperature environment. Melon (Cucumis melo) is sensitive to low temperature, but the genetic pattern for chilling tolerance of melon seedlings remained unclear to date. In the present study, 2 melon inbred lines, which showed tolerance and sensitive to low temperature, were used to develop 6 generation populations for joint analysis, aiming to pinpoint the genetic model controlling chilling tolerance of melon seedlings and function model of the genes related to the trait. For this analysis, a mixed major-gene plus polygenes inheritance model was applied for pursuing the inheritance law of chilling tolerance of melon seedlings in spring and autumn. The results showed that chilling tolerance had typical characters of quantitative traits and tended to inherit dominantly. The optimal genetic pattern fitted 2 pairs of additive-dominance-epitasis major genes plus additive-dominant polygene model. Dominant effects played a main role for the major genes, meanwhile epistatic effects were also observed with these genes. All the gene effects were sensitive to environmental factors. Three generation populations (B1∶2, B2∶2 and F2∶3) had a major gene heritability of 73.22%, 92.36% and 95.57% in spring and 49.58%, 84.15% and 83.18% in autumn, respectively. The heritability of polygene was much low, only being detected in B1∶2 generation. Selection efficiency of major genes was much high in the early generations of F2 and its self-bred progenies as well as the backcross progenies (B2×sensitive parent). B1 generation could be more suitable for selection for polygenes. The present study could provide a reference for genetic improvement of chilling tolerance of melon seedlings.
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