Resistance Allelism Study of Chinese Cabbage (Brassica campestris spp. pekinensis) Turnip mosaic virus Disease
MAN Wei-Ping1,2,*, ZHANG Zhi-Gang2,*, LIU Shuan-Tao2, WANG Rong-Hua2, WANG Li-Hua2, XU Nian-Fang3, XU Wen-Ling2, LIU Xian-Xian2, LIU Chen2, LI Qiao-Yun1,2,**, ZHAO Zhi-Zhong2,**
1 College of Life Sciences, Shandong Normal University, Jinan 250014, China; 2 Vegetable and Flower Institute of Shandong Academy of Agricultural Sciences/Shandong Branch of National Center for Vegetables Improvement/Shandong Key Laboratory for Biology of Greenhouse Vegetables/Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables, Ministry of Agriculture and Rural Affairs, Jinan 250100, China; 3 Agricultural and Sideline Raw Materials Research Institute on Light Industry of Shandong, Gaomi 261500, China
Abstract：Chinese cabbage (Brassica rapa spp. pekinensis) originated in China, and is an important vegetable in China and even in East Asia. Virus disease is one of the most important diseases for Chinese cabbage production, in which Turnip mosaic virus (TuMV) is the main pathogen. The inheritance pattern for TuMV resistance is very complicated in Chinese cabbage. In order to better understand the similarities and differences of resistance sites between different TuMV resistant materials, the high-generation inbred line TuMV resistant material '73' and National TuMV source resistant material '8407' were used as core parents to study the similarities and differences of TuMV resistant sites in 9 resistant materials ('73', '8407', 'Zao 219', '07-372', C07-14', 'Henan 304', '322', '826' and '07-55'). By crossing and inbreeding, F1 and F2 populations of 'Zao219'×'8407', '07-372'×'8407', '07-14'×'8407', 'Henan304'×'8407', '322'×'8407', '826'×'8407', '07-55'×'8407', '73'×'8407', 'Zao219'×'73', '07-372'×'73', 'Henan304'×'73', '322'×'73', '07-55'×'73', '07-372'×'322', 'Henan304'×'322', '07-372'×'Henan304', 'Zao219'×'322', 'Zao219'×'Henan304' and 'Zao219'×'07-372' were constructed. The C4 strains of TuMV was inoculated on the above F1, F2 and parent materials. The similarities and differences of resistance sites among different combinations were judged according to the resistance performance of F1 and the resistance isolation of F2. The results showed, in combinations '73'×'8407' and '322'×'8407', both F1 were resistant, individual susceptible plants were found in both F2 populations, indicating that the resistance sites in '73' and '322' were linked to that in '8407' but not allelic. In combination '07-55'×'8407', F1 was resistant, no susceptible plants were found in F2, but 3 grade 1 and 6 grade 3 plants, indicated that the resistance site in '07-55' and '8407' were incomplete allelic, should be linked. In combinations '322'×'73' and '07-55'×'73', F1 and all of F2 plants were resistant, no susceptible plants were found in F2, indicating that the resistance sites of '322', '07-55' and '8407' were allelic, and their resistance was controlled by the same gene. In combinations 'Zao219'×'8407', '07-372'×'8407', '07-14'×'8407', and '826'×'8407', all of the F1 and F2 plants were resistant, no susceptible plants were found in F2, indicating that the resistance sites of 'Zao219', '07-372', '07-14', '826' and '8407' were allelic, and their resistance were controlled by the same gene. In combination 'Henan304'×'8407', F1 was susceptible, both resistant and susceptible plants were found in F2, and the segregation ratio of resistant and susceptible plants was 9∶7, indicating that the resistance sites of 'Henan304' and '8407' were not allelic, TuMV resistant genes in 'Henan304' and '8407' were complementary. In combination 'Henan304'×'322', F1 was resistant, both resistant and susceptible plants were found in F2, and the segregation ratio of resistant and susceptible plants was 15∶1, indicated that the resistance sites of 'Henan304' and '322' were not allelic, TuMV resistant genes in 'Henan304' and '322' were inherited independently. This result provides a basis for the next step of targeted selection of disease resistant materials and research on the molecular markers of resistance to TuMV in Chinese cabbage. It also broadens the germplasm resources of resistance of Chinese cabbage for TuMV resistant breeding, and provides theoretical guidance for the selection of parents and offspring of resistance breeding of Chinese cabbage to TuMV.
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