主要大白菜品种及种质资源在eIF(iso)4E.a和eIF(iso)4E.c位点的基因型精准鉴定

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摘要摘要由芜菁花叶病毒(Turnip mosaic virus, TuMV)引起的病毒病是大白菜(Brassica rapa ssp. pekinensis)主要病害之一。大白菜中野生型eIF(iso)4E.a (A)和eIF(iso)4E.c (C)共同介导病毒的早期侵染，单个或两个基因发生功能缺失突变将使病毒难以侵染植株，大白菜表现为抗病毒表型。前人已筛选出eIF(iso)4E.a的可变剪切突变(a1)和假基因突变(a2)、eIF(iso)4E.c的移码突变(c1)和转座子插入突变(c2)。本研究对3种eIF(iso)4E.a等位基因序列进行了重新分析和评估，优化了检测标记，结合本课题组已经获得的鉴定eIF(iso)4E.c等位突变的标记，对中国市场销售的38个大白菜品种和本课题组创制的96份叠抱类大白菜种质资源进行了筛查。结果表明，A、a1和a2基因型在市售大白菜杂交种和叠抱类种质资源中的检出频率分别是36.8%、40.8%、22.4%和26.1%、28.1%、45.8%。a1和a2两种突变等位基因的检出频率总体达71.3%。相比之下，在eIF(iso)4E.c位点，供试的134份材料中仅有1份种质是c1c1基因型、13份种质是c2c2基因型，两种突变等位基因所占的比例仅为10.4%。本研究结果为利用抗病毒功能基因进行种质鉴定和新品种培育提供了可选的标记，对已有品种的推广提供了抗病毒分子证据。

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	刘栓桃
	张志刚
	王立华
	王荣花
	李巧云
	陈伟
	赵智中
	张全芳
	梁水美
	王淑芬

关键词 ：大白菜, 主栽品种, 种质资源, eIF(iso)4E.a和eIF(iso)4E.c位点, 等位变异

Abstract：Abstract Virus disease caused by Turnip mosaic virus (TuMV) is one of the most serious problems during Chinese cabbage (Brassica rapa ssp. pekinensis) cultivation. Initial infection of TuMV in Chinese cabbage has been demonstrated to be related to wild type BraA.eIF(iso)4E.a (A) and BraA.eIF(iso)4E.c (C). Loss-of-function mutation in one or two genes hinders TuMV infection and results in virus-resistant phynotype in Chinese cabbage. Two kind of allelic variations, miss splicing mutation (a1) and psedogene mutation (a2) in eIF(iso)4E.a, frame-shift mutation (c1) and transposon-insertion mutation (c2). in eIF(iso)4E.c, has been identified respectively from Chinese cabbage. In this study, the differences among three kinds of eIF(iso)4E.a genotype were compared again. Simple and convenient markers were developed. The precise genotyping of eIF(iso)4E.a and eIF(iso)4E.c was detected among 38 hybrids bought from seed market and 96 germplasms created by our breeding team. The results showed that the allele frequencies for A, a1 and a2 were 36.8%, 40.8%, 22.4% among hybrids and 26.1%, 28.1%, 45.8% among germplasms respectively. Total genotype frequency for two mutant alleles of a1 and a2 was 70.1%. Comparatively, only one germplasm carried c1c1 genotype and 13 germplasms carried c2c2 genotype. The total genotype frequency for c1 and c2 was only 10.4%. The results provide molecular markers for utilizing the genes in germplasms detection and new vriaties breeding. They also provide molecular evidence for continuing expanding of those virus-resistance hybrids.

Key words： Chines cabbage Main hybrids Germplasmes eIF(iso)4E.a and eIF(iso)4E.c Allele variations

收稿日期: 2017-06-30 出版日期: 2018-05-02

ZTFLH:

S643.1

基金资助:山东省重点研发计划;山东省自然科学基金;山东省农业科学院创新工程

通讯作者: 赵智中 E-mail: zhaozhizhong454@163.com

引用本文:

刘栓桃张志刚王立华王荣花李巧云陈伟赵智中张全芳梁水美王淑芬. 主要大白菜品种及种质资源在eIF(iso)4E.a和eIF(iso)4E.c位点的基因型精准鉴定[J]. , 2018, 26(5): 888-898.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I5/888

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