Identification of OFP Gene Family and Their Expression Analysis Under Low-temperature Stress in Wheat (Triticum aestivum)
XU Ke1, WANG Wei-Wei1,2, WU Ning-Jing1, ZHANG Shu-Hua1, ZHAO Yong1,*, YANG Xue-Ju1,*
1 College of Agronomy/State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China; 2 Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou 061001, China
Abstract：Ovate family proteins (OFPs) is a plant-specific transcription factor gene family, which plays an important role in low temperature stress response. In order to explore the OFP gene family members in wheat (Triticum aestivum), as well as the expression characteristics under cold stress, in this study, the members of the OFP family were identified and bioinformatics analysis was carried out in the whole genome of wheat, the expression pattern of TaOFPs in different tissues under low temperature stress were analyzed based on RNA-seq. The results showed that a total of 100 TaOFPs were identified, which were evenly distributed on A, B and D subgenomes. Wheat, rice (Oryza sativa) and Arabidopsis OFPs were divided into 6 subgroups based evolutionary analysis, and the OFP genes in gramineae were more conservative during evolution. The results of promoter elements analysis indicated that a large number of hormone related and abiotic stress response cis-acting elements, such as MeJA responsive elements, ABA responsive elements, low temperature responsive elements and drought responsive elements existed in promoter sequences of TaOFPs. The expression analysis revealed the tissue and stage specific expression patterns of TaOFPs. Under freezing stress, 25 TaOFPs were differentially expressed in cold acclimation and freezing specificity, TaOFP4-1D, TaOFP10-2B, TaOFP27-4D, TaOFP31-5A and TaOFP33-5D showed opposite expressed pattern in cold acclimation and freezing and un-cold acclimation and freezing treatments, suggested that these genes may involved in regulation of cold resistance of wheat. This study provides reference for further exploring the function of TaOFP family members and analyzing its regulatory mechanism in low-temperature stress.
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